DNA BENDING BY CRO PROTEIN IN SPECIFIC AND NONSPECIFIC COMPLEXES - IMPLICATIONS FOR PROTEIN SITE RECOGNITION AND SPECIFICITY
DNA BENDING BY CRO PROTEIN IN SPECIFIC AND NONSPECIFIC COMPLEXES - IMPLICATIONS FOR PROTEIN SITE RECOGNITION AND SPECIFICITY
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DOI：
10.1126/science.7985026
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发表时间：
1994-12-02
期刊：
影响因子：
56.9
通讯作者：
BUSTAMANTE, C
中科院分区：
文献类型：
--
作者：
ERIE, DA;YANG, GL;BUSTAMANTE, C
扫描力显微镜被用来解决λ Cro蛋白结合作为一个单一的二聚体或多个二聚体的三个操作（O-R）网站。测定Cro与特异性和非特异性位点结合诱导的弯曲角，特异性复合物为69度+/-11度，非特异性复合物为62度+/-23度。非特异性位点的弯曲对于使其特异性位点弯曲的蛋白质如Cro是有利的，因为它增加了蛋白质的结合特异性，并且它可以被蛋白质用于识别其靶序列所需的样品接触。在此提出，非特异性DNA的弯曲可能是DNA结合蛋白中使其特异性位点弯曲的一般性质。
Scanning force microscopy was used to resolve lambda Cro protein when bound as a single dimer or multiple dimers to its three operator (O-R) sites. The bend angles induced by binding of Cro to specific and nonspecific sites were determined and are 69 degrees +/- 11 degrees for specific and 62 degrees +/- 23 degrees for nonspecific complexes. Bending of the nonspecific sites is advantageous for a protein such as Cro that bends its specific site, because it increases the binding specificity of the protein and it can be used by the protein to sample contacts required for the recognition of its target sequence. It is proposed here that bending of nonspecific DNA may be a general property among DNA binding proteins that bend their specific sites.