OptiRNAi, an RNAi design tool
OptiRNAi, an RNAi design tool
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DOI：
10.1016/j.cmpb.2003.09.002
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发表时间：
2004-07-01
影响因子：
6.1
通讯作者：
Duncan, MK
中科院分区：
文献类型：
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作者：
Cui, WW;Ning, JC;Duncan, MK
RNA干扰技术是近年来发展起来的一种反向遗传学手段，与传统的基因敲除方法相比具有许多优点。适当选择与靶基因的特定区域相同的双链RNA对于成功实施该技术至关重要。最近，Elbashir et at. [Methods 26（2002）199]已经建立了siRNA序列选择的经验标准，其显著提高了RNAi尝试的成功率。我们已经开发了OptiRNAi，一种计算工具，它使用Elbashir等。标准来预测用于siRNA产生的适当靶序列。然后，研究者可以使用针对RNAi设计优化的嵌入式Blast搜索引擎来评估这些siRNA对目标靶标的特异性。因此，OptiRNAi是一种高效且用户友好的RNAi设计工具，其标准比其他可用工具更严格。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
RNA interference (RNAi), a recently developed reverse genetics toot, has many advantages compared to traditional gene knockout methods. Appropriate selection of double stranded RNAs identical to a specific region(s) of the target gene is critical for the successful implementation of this technology. Recently, Elbashir et at. [Methods 26 (2002) 199] has established empirical criteria for siRNA sequence selection that significantly improved the success rate for RNAi attempts. We have developed OptiRNAi, a computational tool, which uses the Elbashir et at. criteria to predict appropriate target sequences for siRNA production. Specificity of these siRNAs for the target of interest can then be assessed by the investigator using the embedded Blast search engine optimized for RNAi design. Thus, OptiRNAi is an efficient and user friendly toot for RNAi design based on criteria that are more stringent than other available tools. (C) 2003 Elsevier Ireland Ltd. All rights reserved.