Structural Mapping of a Chaperone- Substrate Interaction Surface**
Structural Mapping of a Chaperone- Substrate Interaction Surface**
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DOI：
10.1002/anie.201310963
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发表时间：
2014-05-12
影响因子：
16.6
通讯作者：
Hiller, Sebastian
中科院分区：
文献类型：
--
作者：
Callon, Morgane;Burmann, Bjoern M.;Hiller, Sebastian
核磁共振波谱被用来检测膜-蛋白质-伴侣复合体中特定位置的分子间短程接触。这是通过一个正交的同位素标记方案实现的，该方案允许明确地检测折叠良好的伴侣和未折叠的底物系之间的分子间NO。这些接触中涉及的残基是伴侣-底物接触界面的一部分。该方法是以70 kDa的细菌Skp-tOmpA复合体为例进行的。
NMR spectroscopy is used to detect site-specific intermolecular short-range contacts in a membrane-protein-chaperone complex. This is achieved by an orthogonal isotope-labeling scheme that permits the unambiguous detection of intermolecular NOEs between the well-folded chaperone and the unfolded substrate ensemble. The residues involved in these contacts are part of the chaperone-substrate contact interface. The approach is demonstrated for the 70kDa bacterial Skp-tOmpA complex.