Cloning and characterization of a novel variant (mM-rdgBβ1) of mouse M-rdgBs, mammalian homologs of Drosophila retinal degeneration B gene proteins, and its mRNA localization in mouse brain in comparison with other M-rdgBs
Cloning and characterization of a novel variant (mM-rdgBβ1) of mouse M-rdgBs, mammalian homologs of Drosophila retinal degeneration B gene proteins, and its mRNA localization in mouse brain in comparison with other M-rdgBs
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DOI：
10.1046/j.1471-4159.2003.01591.x
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发表时间：
2003-02-01
影响因子：
4.7
通讯作者：
Kondo, H
中科院分区：
文献类型：
--
作者：
Takano, N;Owada, Y;Kondo, H
我们报告的克隆，表征和定位在大脑中的一种新的异构体称为mM-rdgBbeta 1（小鼠型的哺乳动物视网膜变性Bbeta 1蛋白）与三个已知的哺乳动物同源物（M-rdgBbeta，M-rdgB 1，M-rdgB 2）的本地化比较。mM-rdgB β 1 cDNA包含119 bp的序列作为已知mM-rdgB β的开放阅读框中的插入形式，并编码269个氨基酸的蛋白质，其计算分子量为31.7 kDa，不同于mM-rdgB β的38.3 kDa的分子量。它还含有一个磷脂酰肌醇转移蛋白（PITP）样结构域类似于已知的三个同源物，以及D-rdgB。重组mM-rdgB β 1蛋白显示出与磷脂酰肌醇的特异性结合活性，但不与其它磷脂结合。这种新的分子不仅位于细胞质中，而且还位于细胞核中，不同于mM-rdgB β的细胞质定位。在原位杂交分析中，mM-rdgB β 1在大脑中的基因表达虽然很弱，但仅限于胚胎阶段，不同于出生前和出生后大脑灰质中mM-rdgB β的广泛表达。综上所述，mM-rdgB β 1被认为在神经发育的磷酸肌醇介导的信号中发挥作用。
We report the cloning, characterization and localization in the brain of a novel isoform termed mM-rdgBbeta1 (mouse type of mammalian retinal degeneration Bbeta1 protein) in comparison with the localization of three known mammalian homologs (M-rdgBbeta, M-rdgB1, M-rdgB2). mM-rdgBbeta1 cDNA contains a sequence of 119 bp as a form of insertion in the open reading frame of the known mM-rdgBbeta , and encodes a protein of 269 amino acids with a calculated molecular mass of 31.7 kDa, different from the molecular mass of 38.3 kDa of mM-rdgBbeta. It also contains a phosphatidylinositol transfer protein (PITP)-like domain similar to the known three homologs, as well as D-rdgB. The recombinant mM-rdgBbeta1 protein shows the specific binding activity to phosphatidylinositol but not to other phospholipids. This novel molecule is localized not only in the cytoplasm but also in the nucleus, different from the cytoplasmic localization of mM-rdgBbeta. In in situ hybridization analysis, the gene expression for mM-rdgBbeta1 in the brain, though weak, is rather confined to the embryonic stage, different from wider expression of mM-rdgBbeta in the gray matters of pre- and post-natal brains. Taken together, mM-rdgBbeta1 is suggested to play a role in the phosphoinositide-mediated signaling in the neural development.