Isolation and characterization of fifteen novel microsatellite loci in golden mandarin fish (Siniperca scherzeri) Steindachne

Isolation and characterization of fifteen novel microsatellite loci in golden mandarin fish (Siniperca scherzeri) Steindachne
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金鳜鱼 (Siniperca scherzeri) Steindachne 中 15 个新微卫星位点的分离和表征

DOI:
10.1007/s12686-012-9601-1
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发表时间:
2012-09-01
影响因子:
1.1
通讯作者:
Yu, Rui
Yu, Rui
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Yang, Min;Liang, Xu-Fang;Yu, Rui

文献摘要

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本文报道了15个新的微卫星位点的分离和鉴定。在34个个体中进行了变异性分析。等位基因多样性范围为2 - 7个等位基因,观察和预期杂合度范围分别为0.294 - 1.000和0.261 - 0.740。经Bonferroni校正后,7个位点显著偏离Hardy-Weinberg平衡,15对位点间未发现显著的连锁不平衡。这是第一个从S. scherzeri。我们希望这些微卫星标记是有用的,在这个物种的群体遗传学研究。
We described the isolation and characterization of 15 new microsatellite loci from golden mandarin fish,Siniperca scherzeri. The analysis of variability was performed in 34 individuals. Allelic diversity ranged from 2 to 7 alleles per locus, with observed and expected heterozygosities ranging from 0.294 to 1.000 and 0.261 to 0.740, respectively. Seven loci deviated significantly from Hardy–Weinberg equilibrium after Bonferroni correction, and no significant linkage disequilibrium were found among 15 pairs of loci following the Bonferroni correction. These are the first microsatellite markers characterized from theS. scherzeri. We expect these microsatellite markers to be useful for population genetic studies in this species.