Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa
Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa
复制标题
编码花粉胞外蛋白的 cDNA 的分子克隆作为甘蓝花粉过敏原的潜在来源
DOI：
10.1016/s0014-5793(98)00174-4
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Masao Watanabe
中科院分区：
文献类型：
--
作者：
K. Toriyama;Kazunori Hanaoka;Takashi Okada;Masao Watanabe
以油菜花粉胞外蛋白为抗原，制备了多克隆抗血清，并用于筛选油菜花药表达cDNA文库。我们获得了五组cDNA克隆，包括与PCP 1、硫氧还蛋白和脂质转运蛋白（LTP）相似的cDNA。显示与LTP序列相似性的cDNA克隆的重组蛋白质被证明结合对卡匹林过敏的患者的IgE。因此，本文报道的cDNA克隆代表了一种新的芜菁花粉过敏原。
A polyclonal antiserum was raised against the extracellular pollen proteins ofBrassica rapaand used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic toBrassicapollen. The cDNA clone reported here, therefore, represents a novel pollen allergen ofBrassica rapa.