A YEAST SMALL NUCLEAR-RNA IS REQUIRED FOR NORMAL PROCESSING OF PRE-RIBOSOMAL RNA
A YEAST SMALL NUCLEAR-RNA IS REQUIRED FOR NORMAL PROCESSING OF PRE-RIBOSOMAL RNA
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DOI：
10.1002/j.1460-2075.1987.tb02763.x
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发表时间：
1987-12-01
期刊：
影响因子：
11.4
通讯作者：
TOLLERVEY, D
中科院分区：
文献类型：
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作者：
TOLLERVEY, D
在酿酒酵母中，在核质RNA丢失的条件下，七种snRNA（snR 3、4、5、8、9、10和17）保留在核中，并且可能是核仁的。所有这些snRNA显示与前核糖体RNA氢键合一致的性质; snR 5和8与20 S前rRNA，snR 3、4、10和17与35 S前rRNA，snR 9与20 S 35 S RNA。缺乏snR 10的菌株在生长中受损，并且在35 S RNA的加工中特别有缺陷。加工减慢，导致35 S RNA积累，并且大多数切割发生在野生型菌株中用于rRNA成熟的后续步骤的位点，而不是在正常位点。
In Saccharomyces cerevisiae, seven snRNAs (snR3, 4, 5, 8, 9, 10 and 17) are retained in the nucleus under conditions in which nucleoplasmic RNAs are lost, and may be nucleolar. All of these snRNAs show properties consistent with hydrogen bonding to pre-ribosomal RNAs; snR5 and 8 with 20S pre-rRNA, snR3, 4, 10 and 17 with 35S pre-rRNA and snR9 with 20.sbd.35S RNA. Strains lacking snR10 are impaired in growth and specifically defective in the processing of 35S RNA. Processing is slowed, leading to 35S RNA accumulation and most cleavage occurs, not at the normal sites, but at sites which in wild-type strains are used for subsequent steps in rRNA maturation.