LncRNA MCM3AP-AS1 Regulates miR-21/PTEN Axis to Affect Cervical Squamous Cell Carcinoma Cell Proliferation and Apoptosis.
LncRNA MCM3AP-AS1 Regulates miR-21/PTEN Axis to Affect Cervical Squamous Cell Carcinoma Cell Proliferation and Apoptosis.
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LncRNA MCM3AP-AS1 调节 miR-21/PTEN 轴影响宫颈鳞状细胞癌细胞增殖和凋亡。
DOI：
10.1615/critreveukaryotgeneexpr.2022041014
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发表时间：
2022
影响因子：
1.6
通讯作者：
Manling Chen
中科院分区：
文献类型：
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作者：
Gang Yan;Zhang Wan;Yechao Zhong;Manling Chen
据报道，LncRNA MCM 3AP-AS 1在乳头状甲状腺癌中上调并发挥致癌作用。然而，对癌症基因组图谱（TCGA）数据集的分析显示，MCM 3AP-AS 1在宫颈鳞状细胞癌（CSCC）中下调。这一观察结果促使我们分析MCM 3AP-AS 1在CSCC中的功能。本研究的研究对象为62例CSCC患者（42 ~ 68岁; 53.7 ± 6.8岁）。根据病历，有51例HPV阳性病例和11例HPV阴性病例。通过RT-qPCR分析基因表达。通过过表达测定，随后通过RT-qPCR和Western印迹探索MCM 3AP-AS 1、miR-21和PTEN之间的相互作用。采用CCK-8细胞增殖分析和细胞凋亡分析方法，研究MCM 3AP-AS 1、miR-21和PTEN在CSCC细胞增殖和凋亡中的作用。我们发现MC-M3 AP-AS 1在CSCC患者中表达下调，其低水平与患者的不良生存密切相关。MCM 3AP-AS 1可与miR-21直接相互作用。然而，miR-21过表达未能影响MCM 3AP-AS 1的表达。有趣的是，MCM 3AP-AS 1过表达降低了PTEN的表达，这是miR-21的靶点。细胞增殖和凋亡分析显示，MCM 3AP-AS 1和PTEN过表达可增加CSCC细胞的凋亡，但抑制其增殖。miR-21过表达发挥相反的作用，并减弱了MCM 3AP-AS 1过表达的作用。因此，MCM 3AP-AS 1可能通过调控miR-21/PTEN轴来调控CSCC细胞增殖和凋亡。
LncRNA MCM3AP-AS1 has been reported to be upregulated and plays an oncogenic role in papillary thyroid cancer. However, analysis of the Cancer Genome Atlas (TCGA) dataset revealed MCM3AP-AS1 downregulation in cervical squamous cell carcinoma (CSCC). This observation encouraged us to analyze the function of MCM3AP-AS1 in CSCC. The research subjects of the present study were 62 CSCC patients (42 to 68 years; 53.7 ± 6.8 years). Based on medical records, there were 51 HPV-positive cases of and 11 HPV-negative cases. Gene expression was analyzed by RT-qPCR. The interactions among MCM3AP-AS1, miR-21, and PTEN were explored by overexpression assays followed by RT-qPCR and Western blot. CCK-8 cell proliferation analysis and cell apoptosis analysis were applied to study the roles of MCM3AP-AS1, miR-21, and PTEN in regulating cell proliferation and apoptosis in CSCC. We found that MC-M3AP-AS1 was downregulated in CSCC patients, and its low level was closely correlated with patients' poor survival. MCM3AP-AS1 could directly interact with miR-21. However, miR-21 overexpression failed to affect MCM3AP-AS1 expression. Interestingly, MCM3AP-AS1 overexpression decreased the expression of PTEN, which is a target of miR-21. Cell proliferation and apoptosis analysis showed that MCM3AP-AS1 and PTEN overexpression increased apoptosis but decreased proliferation of CSCC cells. MiR-21 overexpression played an opposite role and attenuated the effects of MCM3AP-AS1 overexpression. Therefore, MCM3AP-AS1 may regulate the miR-21/PTEN axis to regulate CSCC cell proliferation and apoptosis.