PRODUCTION OF IMMORTALIZED DISTAL RESPIRATORY EPITHELIAL-CELL LINES FROM SURFACTANT PROTEIN-C SIMIAN VIRUS-40 LARGE TUMOR-ANTIGEN TRANSGENIC MICE
PRODUCTION OF IMMORTALIZED DISTAL RESPIRATORY EPITHELIAL-CELL LINES FROM SURFACTANT PROTEIN-C SIMIAN VIRUS-40 LARGE TUMOR-ANTIGEN TRANSGENIC MICE
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DOI：
10.1073/pnas.90.23.11029
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发表时间：
1993-12-01
影响因子：
11.1
通讯作者：
WHITSETT, JA
中科院分区：
文献类型：
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作者：
WIKENHEISER, KA;VORBROKER, DK;WHITSETT, JA
在人表面活性蛋白C(SP-C)基因启动子区域的转录调控下，从含有病毒癌基因猴病毒40(SV40)大肿瘤抗原的转基因小鼠中获得代表远端细支气管和肺泡上皮的小鼠肺上皮(MLE)细胞系。培养30-40代，细胞生长迅速，无接触抑制，细胞形态为上皮细胞。部分MLE细胞系在电子显微镜下可见微绒毛、胞质多泡体和多板层包涵体(肺泡II型细胞的形态特征)。MLE细胞还保持了远端呼吸道上皮细胞的功能特征，包括表面活性蛋白和mRNAs的表达以及分泌磷脂的能力。所有细胞系均检测到外源性SV40大肿瘤抗原基因的表达。SP-C/SV40大肿瘤抗原转基因小鼠和MLE细胞系将为肺表面活性物质的产生和调控以及肺发育和肿瘤发生的研究奠定基础。
Murine lung epithelial (MLE) cell lines representing the distal bronchiolar and alveolar epithelium were produced from lung tumors generated in transgenic mice harboring the viral oncogene simian virus 40 (SV40) large tumor antigen under transcriptional control of a promoter region from the human surfactant protein C (SP-C) gene. The cell lines exhibited rapid growth, lack of contact inhibition, and an epithelial cell morphology for 30-40 passages in culture. Microvilli, cytoplasmic multivesicular bodies, and multilamellar inclusion bodies (morphologic characteristics of alveolar type II cells) were detected in some of the MLE cell lines by electron microscopic analysis. The MLE cells also maintained functional characteristics of distal respiratory epithelial cells including the expression of surfactant proteins and mRNAs and the ability to secrete phospholipids. Expression of the exogenous SV40 large tumor antigen gene was detected in all of the generated cell lines. The SP-C/SV40 large tumor antigen transgenic mice and the MLE cell lines will be useful for the study of pulmonary surfactant production and regulation as well as lung development and tumorigenesis.