Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress
Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress
复制标题
恶臭假单胞菌 ND6 萘诱导应激细胞蛋白质组变化的定量分析
DOI：
10.1007/s10529-015-1828-y
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发表时间：
2015-08-01
影响因子：
2.7
通讯作者：
Zhao, Hua-Bing
中科院分区：
文献类型：
--
作者：
Li, Shan-Shan;Hu, Xiao;Zhao, Hua-Bing
目的探讨恶臭假单胞菌对萘的耐受是否与nahAaAbAcAdBFCED和nahGTHINLOMKJ操纵子以外的基因有关。采用定量蛋白质组学方法研究了2和4 g萘/l培养的恶臭假单胞菌ND6的细胞反应。蛋白质组学数据的比较分析发现，参与热休克和普遍应激反应、萘降解、包膜合成和运动的22种蛋白质的表达水平上调；而参与蛋白质和脂肪酸合成、碳化合物、核苷酸和氨基酸代谢以及小分子运输的26个蛋白表达水平下调。我们的发现提供了在蛋白质水平上对高萘浓度的恶臭假单胞菌的细胞反应的见解。
To investigate whether genes other than the operons nahAaAbAcAdBFCED and nahGTHINLOMKJ of Pseudomonas putida are involved in the tolerance of the bacterium to naphthalene.Cellular responses of P. putida ND6 grown with 2 and 4 g naphthalene/l were investigated using a quantitative proteomic-based approach. Comparative analysis of the proteome data identified that the expression levels of 22 proteins involved in heat shock and universal stress response, naphthalene degradation, cell envelope synthesis, and motility were up-regulated; while the expression levels of 26 proteins involved in protein and fatty acid synthesis, carbon compound, nucleotide, and amino acid metabolism, and small molecule transport were down-regulated.Our findings offer insights into the cellular response of P. putida to high naphthalene concentrations at the protein level.