Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis
Transgenic Rice Plants Expressing a Modified cry1Ca1 Gene are Resistant to Spodoptera litura and Chilo suppressalis
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表达修饰的cry1Ca1基因的转基因水稻植物可抵抗斜纹夜蛾和二化螟
DOI：
10.1007/s12033-009-9201-9
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发表时间：
2009-11-01
影响因子：
2.6
通讯作者：
Altosaar, Illimar
中科院分区：
文献类型：
--
作者：
Zaidi, Mohsin Abbas;Ye, Gongyin;Altosaar, Illimar
根据水稻基因的密码子使用情况，对苏云金芽孢杆菌杀虫蛋白Cry 1Ca 1的编码序列进行了大范围的改造。合成的cry 1Ca 1编码序列的总体G + C含量提高到65%，具有富集单子叶植物优选的G和C末端密码子的额外偏差。利用农杆菌介导法将该基因导入粳稻品种秀水11。转基因水稻对二化螟和斜纹夜蛾幼虫具有很高的抗性。在转基因水稻叶片中获得了高水平的Cry 1Ca 1蛋白，其有效地实现了对S. litura和C.二化螟幼虫Cry 1Ca 1在转基因植株叶片中的表达量占可溶性蛋白总量的0.34%。C. suppressalis和S.斜纹夜蛾最大可取食转基因叶片面积为1.89和4.89 mm（2），而这些幼虫取食非转基因叶片的面积则明显更大，分别为58.33和61.22 mm（2）。对转基因R-1植株的分析表明，cry 1Ca 1基因在后代植株中得到了遗传，并对黄瓜枯萎病提供了完全的保护作用。suppressalis和S.斜纹夜蛾幼虫
Nucleotide sequence encoding the truncated insecticidal Cry1Ca1 protein from Bacillus thuringiensis was extensively modified based on the codon usage of rice genes. The overall G + C contents of the synthetic cry1Ca1 coding sequence were raised to 65% with an additional bias of enriching for G and C ending codons as preferred by monocots. The synthetic gene was introduced into the Chinese japonica variety, Xiushui 11, by Agrobacterium-mediated transformation. Transgenic rice plants harboring this gene were highly resistant to Chilo suppressalis and Spodoptera litura larvae as revealed by insect bioassays. High levels of Cry1Ca1 protein were obtained in the leaves of transgenic rice, which were effective in achieving 100% mortality of S. litura and C. suppressalis larvae. The levels of Cry1Ca1 expression in the leaves of these transgenic plants were up to 0.34% of the total soluble proteins. The larvae of C. suppressalis and S. litura could consume a maximum of 1.89 and 4.89 mm(2) of transgenic leaf area whereas the consumption of nontransgenic leaves by these larvae was significantly higher; 58.33 and 61.22 mm(2), respectively. Analysis of R-1 transgenic plants indicated that the cry1Ca1 was inherited by the progeny plants and provided complete protection against C. suppressalis and S. litura larvae.