Myosin heavy chain composition of single fibres from normal human muscle.
Myosin heavy chain composition of single fibres from normal human muscle.
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肌球蛋白重链由来自正常人体肌肉的单纤维组成。
DOI：
10.1042/bj2500307
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Giovanni Salviati
中科院分区：
文献类型：
--
作者：
D. Biral;R. Betto;Daniela DANIELI;Giovanni Salviati
在存在 33% 甘油的情况下，对来自正常人肌肉的纯化肌球蛋白进行电泳分析，显示出三个不同的蛋白条带，通过亲和纯化的多克隆抗体将其鉴定为 1、2B 和 2A 型肌球蛋白重链 (MHC) 亚型。对人体单个肌纤维的 MHC 分析表明，人体肌肉含有大量纤维，显示 2A 型和 2B 型 MHC 共存。
Electrophoretic analysis in the presence of 33% glycerol of purified myosin from normal human muscle shows three distinct protein bands which are identified as type 1, 2B, and 2A myosin heavy chain (MHC) isoforms by affinity-purified polyclonal antibodies. Analysis of MHC of single human muscle fibres shows that human muscles contain a large population of fibres showing the coexistence of type 2A and 2B MHC.