A microRNA-initiated DNAzyme motor operating in living cells.
A microRNA-initiated DNAzyme motor operating in living cells.
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DOI：
10.1038/ncomms14378
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发表时间：
2017-03-06
影响因子：
16.6
通讯作者：
Le XC
中科院分区：
文献类型：
--
作者：
Peng H;Li XF;Zhang H;Le XC
合成DNA马达具有模仿细胞中天然蛋白质马达的巨大潜力，但合成DNA马达在活细胞中的操作仍然具有挑战性，并且尚未得到证实。在这里，我们报告了一个DNAzyme马达，在活细胞中响应特定的细胞内目标。整个马达系统构建在一个20 nm的金纳米颗粒（AuNP）上，该纳米颗粒装饰有数百个作为DNA轨道的底物链和数十个DNA酶分子，每个DNA酶分子都被一个锁定链沉默。靶分子与马达系统的细胞内相互作用启动了AuNP上马达的自主行走。响应于特定微小RNA的示例性DNA酶马达使得能够扩增检测个体癌细胞中的特定微小RNA。由特定的细胞内靶点激活，这些自供电的DNAzyme马达将在生物功能的控制和调节中具有不同的应用。合成DNA纳米机器已被设计用于在体外执行各种任务。在这里，作者建立了一个纳米粒子系统，将DNAzyme和DNA轨道集成在金纳米粒子上，以促进细胞摄取，并将其作为活细胞中的实时miRNA成像工具。
Synthetic DNA motors have great potential to mimic natural protein motors in cells but the operation of synthetic DNA motors in living cells remains challenging and has not been demonstrated. Here we report a DNAzyme motor that operates in living cells in response to a specific intracellular target. The whole motor system is constructed on a 20 nm gold nanoparticle (AuNP) decorated with hundreds of substrate strands serving as DNA tracks and dozens of DNAzyme molecules each silenced by a locking strand. Intracellular interaction of a target molecule with the motor system initiates the autonomous walking of the motor on the AuNP. An example DNAzyme motor responsive to a specific microRNA enables amplified detection of the specific microRNA in individual cancer cells. Activated by specific intracellular targets, these self-powered DNAzyme motors will have diverse applications in the control and modulation of biological functions. Synthetic DNA nanomachines have been designed to perform a variety of tasks in vitro. Here, the authors build a nanomotor system that integrates a DNAzyme and DNA track on a gold nanoparticle, to facilitate cellular uptake, and apply it as a real-time miRNA imaging tool in living cells.