PROTEIN STABILITY CURVES
PROTEIN STABILITY CURVES
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DOI：
10.1002/bip.360261104
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发表时间：
1987-11-01
期刊：
影响因子：
2.9
通讯作者：
SCHELLMAN, JA
中科院分区：
文献类型：
--
作者：
BECKTEL, WJ;SCHELLMAN, JA
蛋白质的稳定性曲线定义为解折叠自由能随温度变化的曲线。对于大多数蛋白质，变性或解折叠时热容的变化很大，但近似恒定。当去折叠是双态过程时，蛋白质稳定性曲线的大多数显著特征可以从这一事实推导出来。得到了一些关系式，包括低温变性的特殊性，稳定性最大值的性质，以及蛋白质特征温度之间的相互关系。该文件关闭一个公式，允许一个计算小的变化，从蛋白质的熔化温度的变化稳定自由能。
The stability curve of a protein is defined as the plot of the free energy of unfolding as a function of temperature. For most proteins the change in heat capacity on denaturation, or unfolding, is large but approximately constant. When unfolding is s two-state process, most of the salient features of the stability curves of proteins can be derived from this fact. A number of relations are obtained, including the special features of low-temperature denaturation, the properties of the maximum in stability, and the interrelationships of the characteristic temperatures of the protein. The paper closes with a formula that permits one to calculate small changes in stabilization free energy from changes in the melting temperature of the protein.