Compositional analysis of chondroitin/dermatan sulfate in rhesus monkeys (Macaca mulatta)
Compositional analysis of chondroitin/dermatan sulfate in rhesus monkeys (Macaca mulatta)
复制标题
恒河猴（Macaca mulatta）软骨素/硫酸皮肤素的成分分析
DOI：
10.1093/bbb/zbac057
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hosaka Yoshinao Z
中科院分区：
文献类型：
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作者：
Cahyadi Danang Dwi;Okamoto Munehiro;Warita Katsuhiko;Takeda-Okuda Naoko;Tamura Jun-ichi;Hosaka Yoshinao Z
糖胺聚糖（GAGs）存在于多种组织中，参与多种生理功能。由于恒河猴（Macaca mulatta）是生物医学研究中应用最广泛的非人灵长类动物，因此了解其组织中gag的组成非常重要。本研究的目的是测定恒河猴几种组织中双糖的含量和硫酸化模式。从雌性和雄性恒河猴的多个组织中提取硫酸软骨素(CS)/硫酸皮聚糖（DS）杂交链。用软骨素酶ABC和ACI消化后进行成分分析，以揭示CS/DS杂交链的硫酸化模式。本研究发现，组织中存在的主要CS/DS双糖单位为A型和C型。E型和iE型不仅在气管组织中有特异性分布，在胃肠道组织中也有特异性分布。
Glycosaminoglycans (GAGs) are found in various tissues and are involved in many physiological functions. Since the rhesus monkey (Macaca mulatta) is the most widely used nonhuman primate in biomedical research, an understanding of the compositions of GAGs in their tissues is important. The aim of this study was to determine the content and sulfation pattern of disaccharides contained in several tissues of the rhesus monkey. The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from several tissues of female and male rhesus monkeys. Compositional analysis was performed after digestion with chondroitinases ABC and ACI to reveal the sulfation pattern of the CS/DS hybrid chain. This study revealed that the major CS/DS disaccharide units present in the tissues were A and C types. The E and iE types were specifically distributed not only in the tracheal tissue but also in gastrointestinal tissues.