In Vitro and in Vivo Assays for Testing Retinoids Effect on Intestinal Progenitors' Lineage Commitments.
In Vitro and in Vivo Assays for Testing Retinoids Effect on Intestinal Progenitors' Lineage Commitments.
复制标题
用于测试类维生素A对肠祖细胞谱系承诺影响的体外和体内测定。
DOI：
10.1007/978-1-0716-3076-1_5
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Gajera KR
中科院分区：
文献类型：
--
作者：
Gajera KR
肠由上皮细胞组成，被复杂的环境包围，如间充质细胞和肠道微生物群。凭借其令人印象深刻的干细胞再生能力，肠道能够不断补充因细胞凋亡或食物通过磨损而丢失的细胞。在过去的十年中，研究人员已经确定了参与干细胞稳态的信号通路，如类维生素A通路。类维生素A还参与健康细胞和癌细胞的细胞分化。在这项研究中，我们描述了几种方法在体外和体内进一步研究维甲酸对干细胞，祖细胞和分化的肠细胞的影响。
The intestine consists of epithelial cells surrounded by a complex environment as mesenchymal cells and the gut microbiota. With its impressive stem cell regeneration capability, the intestine is able to constantly replenish cells lost through apoptosis or abrasion by food passing through. Over the past decade, researchers have identified signaling pathways involved in stem cell homeostasis such as retinoids pathway. Retinoids are also involved in cell differentiation of healthy and cancer cells. In this study, we describe several approaches in vitro and in vivo to further investigate the effect of retinoids on stem cells, progenitors, and differentiated intestinal cells.