SDH2 is involved in proper hypha formation and virulence in Candida albicans
SDH2 is involved in proper hypha formation and virulence in Candida albicans
复制标题
SDH2 参与白色念珠菌的正常菌丝形成和毒力。
DOI：
10.2217/fmb-2018-0033
复制
发表时间：
2018-08-01
影响因子：
3.1
通讯作者：
Wang, Yan
中科院分区：
文献类型：
--
作者：
Bi, Shuang;Lv, Quan-Zhen;Wang, Yan
目的
探讨SDH 2在白念珠菌产毒过程中的作用。
材料和方法
使用秀丽隐杆线虫和小鼠念珠菌病模型来评估sdh 2 Δ/Δ突变体的毒力。采用不同的菌丝诱导培养基对该菌的菌丝生长进行了研究。白色念珠菌DCFH-DA用于测量细胞内活性氧（ROS）水平。
结果
sdh 2 Δ/Δ突变株在C. elegans模型，在鼠念珠菌病模型中低毒性，并且在体外和体内都有缺陷以形成细丝。sdh 2 Δ/Δ突变体细胞内活性氧水平升高，sdh 2 Δ/Δ的表达缺陷可通过降低细胞内活性氧水平而得到修复。
结论
SDH 2在C.白念珠菌的致病性和毒力可能是通过影响细胞内活性氧来实现的。[公式：见正文]。
AIM
To investigate the role of SDH2 in Candida albicans filamentation and virulence.
MATERIALS & METHODS
Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels.
RESULTS
The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS.
CONCLUSION
SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. [Formula: see text].