High-resolution NMR spectroscopy of membrane proteins in aligned bicelles

High-resolution NMR spectroscopy of membrane proteins in aligned bicelles
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DOI:
10.1021/ja045631y
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发表时间:
2004-12-01
影响因子:
15
通讯作者:
Opella, SJ
Opella, SJ
中科院分区:
化学1区
文献类型:
--
作者:
De Angelis, AA;Nevzorov, AA;Opella, SJ

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在磁性排列的双胞中,15N标记的膜蛋白可以获得高分辨率的固体核磁共振谱。快速单轴向扩散的双层正常的结果在单线光谱,包含蛋白质结构测定所需的取向信息。
High-resolution solid-state NMR spectra can be obtained from uniformly15N-labeled membrane proteins in magnetically aligned bicelles. Fast uniaxial diffusion about the axis of the bilayer normal results in single-line spectra that contain the orientational information necessary for protein structure determination.