Angiotensin II and Angiotensin III in Rat Blood
Angiotensin II and Angiotensin III in Rat Blood
复制标题
大鼠血液中的血管紧张素 II 和血管紧张素 III
作者：
P. Semple;J. Morton
在正常大鼠的动脉和静脉血浆中测定血管紧张素II及其代谢产物[des-Asp 1]-血管紧张素II（血管紧张素III）、[des-（Asp 1，Arg 2）]血管紧张素II和[des-（Asp 1，Arg 2，Val 3）]血管紧张素II。使用纸层析分离肽，所述肽使用与这些代谢物中的每一种具有100%交叉反应的血管紧张素II抗血清用放射免疫测定法测量。动脉血浆中平均33%的免疫反应性物质为血管紧张素II，58%为血管紧张素HI，9%为[des-（Asp 1，Arg 2）]血管紧张素II/[des-（Asp 1，Arg 2，Val 3）]血管紧张素II。在静脉血浆中发现了类似的分布。这和其他证据表明，血管紧张素诱导的醛固酮分泌大鼠，不像其他哺乳动物物种迄今为止研究，可能是由血管紧张素III介导的。
Angiotensin II and its metabolites, [des-Asp1]-angiotensin II (angiotensin III), [des-(Asp1,Arg2)]angiotensin II, and [des-(Asp1, Arg2, Val3)]angiotensin II, were measured in arterial and venous plasma from normal rats. Paper chromatography was used to separate the peptides which were measured with a radioimmunoassay method using an angiotensin II antiserum which had a 100% cross-reaction with each of these metabolites. A mean of 33% of the immunoreactive material in arterial plasma was angiotensin II, 58% angiotensin HI, and 9% [des-(Asp1, Arg2)]angiotensin II/[des-(Asp1, Arg2,Val3)]angiotensin II. A similar distribution was found in venous plasma. This and other evidence indicates that angiotensin-induced aldosterone secretion in the rat, unlike that in other mammalian species so far studied, may be mediated by angiotensin III.