Hydrodynamic relaxation using stopless flow injection in split inlet sedimentation field-flow fractionation.
Hydrodynamic relaxation using stopless flow injection in split inlet sedimentation field-flow fractionation.
复制标题
在分流入口沉降场流分馏中使用不间断流动注射进行流体动力学松弛。
DOI：
10.1021/ac00196a023
复制
发表时间：
1989
影响因子：
7.4
通讯作者：
Giddings,JC
中科院分区：
文献类型：
--
作者：
Lee,SH;Myers,MN;Giddings,JC
在槽道流动恢复之前，用于样品松弛(其中样品颗粒接近于一个壁面的平衡)的注入。如果不停止通道流动(无步骤流动程序)，则由于在再松弛过程中颗粒的向下迁移，洗脱轮廓被移动和扭曲。为了避免峰值失真，同时保留无分流程序的优点，通道入口的物理分离器将进入的流动流分成两个子流；样品仅注入其中一个子流。通过这种方式，实现了快速的(虽然不是完全的)流体动力松弛。将这种无分流注射程序与使用亚微米(普通FFF)和超微米(STRLC FFF)聚苯乙烯胶乳颗粒的普通停流和无分流程序进行了比较。研究发现，这种新的工艺消除了通常伴随着无弯头流动注射的大部分变形。然而，还需要进一步的优化，以匹配停止流法的高分辨率。
Injection for sample relaxation (In which the sample particles approach equilibrium near one wall) before the resumptionof channel flow. If the channel flow Is not stopped (stopless flow procedure), the elution profile Is shifted and distorted due to the downstream migration of the particles during the re-laxation process. To avoid peak distortion while retaining the advantages of the stopless flow procedure, a physical splitter at the channel Inlet divides the enteringflow stream Into two substreams; sample Is Injected Into only one of these. In this way a rapid (although not complete) hydrodynamic relaxation Is realized. This stopless split flow Injection procedure Is compared to ordinary stop and stopless flow procedures using both submicrometer (normal FFF) and supramicrometer (sterlc FFF) polystyrene latex particles. It Is found that much of the distortion normally accompanying stopless flow Injection Is eliminated by this new procedure. However, further optimization is needed to match the high resolution of the stop flow method.