Caldesmon is a Ca2+-regulatory component of native smooth-muscle thin filaments.
Caldesmon is a Ca2+-regulatory component of native smooth-muscle thin filaments.
复制标题
Caldesmon 是天然平滑肌细丝的 Ca2 调节成分。
DOI：
10.1042/bj2310517
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
W. Lehman
中科院分区：
文献类型：
--
作者：
Steven B Marston;W. Lehman
四种平滑肌类型（砂囊，胃，气管，主动脉）的细丝制剂都激活肌球蛋白MgATP酶活性，由Ca 2+调节，并含有肌动蛋白，原肌球蛋白和120000-140000-Mr蛋白的摩尔比例为1：1/7：1/26。来自所有来源的120000-140000-Mr蛋白是肌动球蛋白ATP酶活性的有效抑制剂。肽图和免疫学证据表明，它是相同的钙调素。定量免疫学数据表明，钙调素是所有细丝的组成部分，细丝结合的钙调素占完整组织中的所有钙调素。发现细丝制剂的肌球蛋白轻链激酶含量可以忽略不计。我们建议，钙调素为基础的细丝Ca 2+调节是一种生理机制，在所有的平滑肌。
Thin-filament preparations from four smooth muscle types (gizzard, stomach, trachea, aorta) all activate myosin MgATPase activity, are regulated by Ca2+, and contain actin, tropomyosin and a 120000-140000-Mr protein in the molar proportions 1:1/7:1/26. The 120000-140000-Mr protein from all sources is a potent inhibitor of actomyosin ATPase activity. Peptide-mapping and immunological evidence is presented showing that it is identical with caldesmon. Quantitative immunological data suggest that caldesmon is a component of all the thin filaments and that the thin-filament-bound caldesmon accounts for all the caldesmon in intact tissue. The myosin light-chain kinase content of thin-filament preparations was found to be negligible. We propose that caldesmon-based thin-filament Ca2+ regulation is a physiological mechanism in all smooth muscles.