The pressure-dependence of the size of extruded vesicles
The pressure-dependence of the size of extruded vesicles
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DOI：
10.1016/s0006-3495(03)74538-x
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发表时间：
2003-08-01
影响因子：
3.4
通讯作者：
Frisken, BJ
中科院分区：
文献类型：
--
作者：
Patty, PJ;Frisken, BJ
通过小孔挤出形成的囊泡的大小的变化进行了讨论，在一个简单的模型。我们的模型预测，半径应作为所施加的压力的平方根，与在各种条件下挤出的囊泡的数据一致。该模型还预测依赖于所使用的孔径和被挤出的囊泡的裂解张力，这与我们的数据一致。通过使用平均孔径范围为50至200 nm的径迹蚀刻聚碳酸酯膜来改变孔径。为了改变溶解张力，研究了由POPC（1-棕榈酰-2-油酰-sn-甘油基-3-磷脂酰胆碱）、POPC和胆固醇的混合物以及POPC和C-16-神经酰胺的混合物制成的囊泡。通过基于挤出的技术测量的POPC：胆固醇囊泡的裂解张力高于纯POPC囊泡的裂解张力，而POPC：神经酰胺囊泡的裂解张力低于POPC囊泡。
Variations in the size of vesicles formed by extrusion through small pores are discussed in terms of a simple model. Our model predicts that the radius should decrease as the square root of the applied pressure, consistent with data for vesicles extruded under various conditions. The model also predicts dependencies on the pore size used and on the lysis tension of the vesicles being extruded that are consistent with our data. The pore size was varied by using track-etched polycarbonate membranes with average pore diameters ranging from 50 to 200 nm. To vary the lysis tension, vesicles made from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine), mixtures of POPC and cholesterol, and mixtures of POPC and C-16-ceramide were studied. The lysis tension, as measured by an extrusion-based technique, of POPC:cholesterol vesicles is higher than that of pure POPC vesicles whereas POPC:ceramide vesicles have lower lysis tensions than POPC vesicles.