Isolation of a mammalian sequence capable of conferring cell cycle regulation to a heterologous gene.
Isolation of a mammalian sequence capable of conferring cell cycle regulation to a heterologous gene.
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能够赋予异源基因细胞周期调节作用的哺乳动物序列的分离。
DOI：
10.1126/science.4059922
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Lee,AS
中科院分区：
文献类型：
--
作者：
Artishevsky,A;Grafsky,A;Lee,AS
构建了含有仓鼠组蛋白H3基因5‘端序列和细菌新霉素抗性基因(Neo)编码序列的杂交基因。该杂合基因导入仓鼠成纤维细胞系K12后，表现出细胞周期依赖性的调控，表现为在细胞周期DNA合成过程中转录产物的最大积累。此外，由于K12温度敏感突变，处于复制前阶段的细胞产生的新信息分子RNA显著减少。
A hybrid gene containing the 5′ sequence of a hamster histone H3 gene and the coding sequence of the bacterial neomycin-resistance gene (neo) was constructed. Upon transfection into the hamster fibroblast cell line K12, the hybrid gene exhibited cell cycle-dependent regulation, as evidenced by the maximal accumulation of theneotranscripts during synthesis of DNA in the cell cycle. In addition, cells arrested in the prereplicative phase, as a consequence of the K12 temperature-sensitive mutation, produced significantly lessneomessenger RNA.