Autologous Chondrocyte Implantation Drives Early Chondrogenesis and Organized Repair in Extensive Full- and Partial-Thickness Cartilage Defects in an Equine Model
Autologous Chondrocyte Implantation Drives Early Chondrogenesis and Organized Repair in Extensive Full- and Partial-Thickness Cartilage Defects in an Equine Model
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DOI：
10.1002/jor.21366
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Matthews, Gloria L.
中科院分区：
文献类型：
--
作者：
Nixon, Alan J.;Begum, Laila;Matthews, Gloria L.
自体软骨细胞植入 (ACI) 已在临床使用超过 15 年，但软骨细胞持久性和对全层病变软骨修复的直接影响的明确证据很少，并且没有关于任何动物模型中部分层缺损的 ACI 的数据。本研究评估了使用骨膜覆盖保护的软骨细胞对马模型部分和全层软骨缺损的影响。在16匹马的髌股关节处制作一对直径15mm的软骨缺损，并单独用ACI或骨膜瓣修复。 8 周时通过临床、显微放射学和组织学外观、II 型胶原蛋白免疫组织化学、蛋白多糖和 DNA 定量来评估疗效。 ACI 改善了部分和全层软骨缺损的组织学评分，包括缺损填充、与下方软骨下骨的附着以及残留软骨细胞积聚的存在。对于部分厚度缺损，软骨细胞优势、II 型胶原含量和甲苯胺染色基质均增强，并且与周围软骨的附着力得到改善。软骨细胞植入提高了移植部分厚度缺损的DNA和PG含量。单独的骨膜补片不能诱导软骨修复。这项研究表明，将软骨细胞植入软骨缺损处可以改善软骨细胞区域的愈合，增强 II 型胶原蛋白的形成，并获得更好的整体软骨愈合分数。在更具挑战性的部分厚度缺损中使用 ACI 也改善了组织学指数和生化含量。马的软骨愈合模型与人类的软骨修复非常相似，这项研究的结果证实了 ACI 后细胞的持久性和早期软骨愈合事件的改善。 (C) 2011 年骨科研究学会。由 Wiley periodicals, Inc. 出版。J Orthop Res 29: 1121-1130, 2011
Autologous chondrocyte implantation (ACI) has been used clinically for over 15 years and yet definitive evidence of chondrocyte persistence and direct impact on cartilage repair in full-thickness lesions is scant and no data are available on ACI in partial-thickness defects in any animal model. This study assessed the effect of chondrocytes secured using periosteal overlay in partial-and full-thickness cartilage defects in the equine model. Paired cartilage defects 15 mm in diameter were made in the patellofemoral joint of 16 horse and repaired with ACI or periosteal flap alone. Response was assessed at 8 weeks by clinical, microradiographic, and histologic appearance, and by collagen type II immunohistochemistry, and proteoglycan and DNA quantification. ACI improved histologic scores in partial-and full-thickness cartilage defects, including defect filling, attachment to the underlying subchondral bone, and presence of residual chondrocyte accumulations. For partial-thickness defects chondrocyte predominance, collagen type II content, and toluidine stained matrix were enhanced, and attachment to the surrounding cartilage improved. DNA and PG content of grafted partial-thickness defects was improved by chondrocyte implantation. Periosteal patches alone did not induce cartilage repair. This study indicated implantation of chondrocytes to cartilage defects improved healing with a combination of persisting chondrocyte regions, enhanced collagen type II formation, and better overall cartilage healing scores. Use of ACI in the more challenging partial-thickness defects also improved histologic indices and biochemical content. The equine model of cartilage healing closely resembles cartilage repair in man, and results of this study confirm cell persistence and improved early cartilage healing events after ACI. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 1121-1130, 2011