Phospholipid polymer–based antibody immobilization for cell-rolling surfaces in stem cell purification system
Phospholipid polymer–based antibody immobilization for cell-rolling surfaces in stem cell purification system
复制标题
基于磷脂聚合物的抗体固定化，用于干细胞纯化系统中的细胞滚动表面
DOI：
10.1080/09205063.2014.936926
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
and T. Yamaoka
中科院分区：
文献类型：
--
作者：
A. Mahara;H. Chen;K. Ishihara;and T. Yamaoka
我们之前开发了一种抗体共轭细胞滚动柱，根据细胞表面标记密度成功分离干细胞亚群，但由于非特异性相互作用，大量注射细胞被保留在柱中。本研究设计了一种具有磷脂极性侧基的两亲共聚物聚[2-甲基丙烯酰氧乙基磷酸胆碱(MPC)-co-n-甲基丙烯酸丁酯(nBMA)-co- n-乙烯基甲酰胺（NVf）]作为新型抗体固定化改性剂。nvf单元的甲酰胺基团转化为活性马来酰亚胺基团。用共聚物包被塑料流动微流控室，固定化降低的抗cd90抗体。将分离的大鼠脂肪组织源性干细胞注射到流动室中，在高速相机显微镜下观察其滚动行为。通过这种固定方法，由于MPC单元，非特异性细胞粘附强烈减少，导致高百分比的滚动细胞。这些结果表明，磷脂极性基团包裹的表面可以用于基于细胞滚动过程的有效干细胞分离系统。
We previously developed an antibody-conjugated cell rolling column that successfully separates stem cell subpopulations depending on the cell surface marker density, but a large amount of the injected cells were retained in the column because of non-specific interactions. In this study, an amphiphilic copolymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (nBMA)-co-N-vinyl formamide (NVf)], with phospholipid polar side groups was designed as a novel antibody-immobilizing modifier. The formamide groups inNVf units were converted to active maleimide groups. A plastic flow microfluidic chamber was coated with the copolymers, and a reduced anti-CD90 antibody was immobilized. The adipose tissue-derived stem cells isolated from the rat were injected into the flow chamber, and their rolling behavior was observed under a microscope with a high-speed camera. Non-specific cell adhesion was reduced strongly by means of this immobilization method because of the MPC unit, resulting in a high percentage of rolling cells. These results demonstrate that a surface coated with phospholipid polar groups can be used in an effective stem cell separation system based on the cell rolling process.