Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel
Genetic Relationship, Virulence Factors, Drug Resistance Profile and Biofilm Formation Ability of Vibrio parahaemolyticus Isolated From Mussel
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DOI：
10.3389/fmicb.2019.00513
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发表时间：
2019-03-20
影响因子：
5.2
通讯作者：
Ha, Sang-Do
中科院分区：
文献类型：
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作者：
Ashrafudoulia, Md;Miza, Md Furkanur Rahaman;Ha, Sang-Do
本研究的目的是探讨副溶血性弧菌分离株的毒力因子、遗传关系、抗生素耐药性以及在30 ℃下对虾和贻贝表面形成生物膜的能力。在这项研究中，8（n = 8）副溶血性弧菌分离贻贝进行了检查。采用聚合酶链反应（PCR）检测不同基因的分布，重复基因外回文PCR（REP PCR）比较遗传关系。纸片扩散法检测细菌对抗生素及多种抗生素的耐药性。采用生物挂膜试验和场发射扫描电镜（FE-SEM）评价生物挂膜能力。透射电镜观察细菌细胞的形态结构。我们的研究结果表明，生物膜相关基因，16 S rRNA，toxR，和tdh，存在于所有测试的副溶血性弧菌分离株（n = 8）。约有62.5%（8株中有5株）的菌株表现出较强的多重耐药指数，平均值为0.56。所有菌株（n = 8）均表现出较强的亲缘关系，并在虾和贻贝表面具有显著的生物膜形成能力。本研究表明，副溶血性弧菌分离株存在毒力因子、较高的多重抗生素耐药指数（MARI）值和有效的生物膜形成能力，可能对人类健康和未来的经济价值构成巨大威胁。还表明，对抗生素的高耐药率可能对治疗副溶血性弧菌感染无效。需要持续监测副溶血性弧菌的抗生素、分子和生物膜特征，以提高海产品安全性。
The objective of this study was to investigate the virulence factors, genetic relationship, antibiotic resistance profile and the biofilm formation ability of Vibrio parahaemolyticus isolates on shrimp and mussel surfaces at 30 degrees C. In this study, eight (n = 8) V. parahaemolyticus isolated from mussel were examined. We used the polymerase chain reaction (PCR) to examine the distribution of different genes, and Repetitive Extragenic Palindromic-PCR (REP-PCR) to compare the genetic relationship. Disk diffusion technique was used to assess antibiotic and multiple-antibiotic resistance. The biofilm formation assay, and field emission scanning electron microscopy (FE-SEM) were used to evaluate biofilm formation ability. Transmission Electron Microscope (TEM) was used to observe the morphological structure of bacterial cell. Our results indicated that the biofilm-associated genes, 16S rRNA, toxR, and tdh, were present in all the tested V parahaemolyticus isolates (n = 8). Approximately, 62.5% (5 isolates among 8 isolates) isolates showed strong multiple-antibiotic resistance index with an average value of 0.56. All isolates (n = 8) showed strong genetic relationship and significant biofilm formation ability on shrimp and mussel surfaces. This study demonstrated that the presence of virulence factors, high multiple antibiotic resistance index (MARI) values, and effective biofilm formation ability of V. parahaemolyticus isolates could be a great threat to human health and economic values in future. It was also suggested that a high resistance rate to antibiotic could be ineffective for treating V. parahaemolyticus infections. The continuous monitoring of V. parahaemolyticus antibiotic, molecular and biofilm characteristics is needed to increase seafood safety.