miR403a and SA Are Involved in NbAGO2 Mediated Antiviral Defenses Against TMV Infection in Nicotiana benthamiana
miR403a and SA Are Involved in NbAGO2 Mediated Antiviral Defenses Against TMV Infection in Nicotiana benthamiana
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miR403a和SA参与NbAGO2介导的烟草TMV感染的抗病毒防御
DOI：
10.3390/genes10070526
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发表时间：
2019-07-01
期刊：
影响因子：
3.5
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
--
作者：
Diao, Pengfei;Zhang, Qimeng;Wuriyanghan, Hada
RNA干扰（RNA interference，RNAi）是植物抵抗病毒感染的一种重要防御反应。植物中RNAi途径的核心机制包括DCL（Dicer Like）、AGO（Argonaute）和RdRp（RNA依赖性RNA聚合酶）。尽管这些RNAi组分参与了拟南芥中的病毒感染反应，但它们对本氏烟草（Nicotiana benthamiana）（植物-病原体相互作用研究的模式植物）中的抗病毒免疫的贡献尚不清楚。在这项研究中，我们研究了N。本塞姆安娜NbAGO 2基因抗TMV（番茄花叶病毒）感染。通过hpRNA构建体的瞬时表达沉默NbAGO 2，在GFP沉默的植物中恢复了GFP（绿色荧光蛋白）的表达，表明NbAGO 2参与了N.本萨米亚纳NbAGO 2的表达被梅萨（水杨酸甲酯）处理和TMV感染转录诱导。通过amiR-NbAGO 2瞬时表达下调NbAGO 2基因损害了植物对TMV感染的抗性。抑制内源性miR 403 a（NbAGO 2的预测调节microRNA）减少了TMV感染。我们的研究为NbAGO 2在烟草中抗烟草花叶病毒TMV的作用提供了证据。benthamiana和SA（水杨酸）通过在TMV感染时诱导NbAGO 2表达来介导这一过程。我们的数据还表明，miR 403 a通过调控靶基因NbAGO 2参与了烟草的TMV防御。本萨米亚娜
RNAi (RNA interference) is an important defense response against virus infection in plants. The core machinery of the RNAi pathway in plants include DCL (Dicer Like), AGO (Argonaute) and RdRp (RNA dependent RNA polymerase). Although involvement of these RNAi components in virus infection responses was demonstrated in Arabidopsis thaliana, their contribution to antiviral immunity in Nicotiana benthamiana, a model plant for plant-pathogen interaction studies, is not well understood. In this study, we investigated the role of N. benthamiana NbAGO2 gene against TMV (Tomato mosaic virus) infection. Silencing of NbAGO2 by transient expression of an hpRNA construct recovered GFP (Green fluorescent protein) expression in GFP-silenced plant, demonstrating that NbAGO2 participated in RNAi process in N. benthamiana. Expression of NbAGO2 was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection. Down-regulation of NbAGO2 gene by amiR-NbAGO2 transient expression compromised plant resistance against TMV infection. Inhibition of endogenous miR403a, a predicted regulatory microRNA of NbAGO2, reduced TMV infection. Our study provides evidence for the antiviral role of NbAGO2 against a Tobamovirus family virus TMV in N. benthamiana, and SA (Salicylic acid) mediates this by induction of NbAGO2 expression upon TMV infection. Our data also highlighted that miR403a was involved in TMV defense by regulation of target NbAGO2 gene in N. Benthamiana.