HISTOCOMPATIBILITY MATCHING VI. MINIATURIZATION OF THE MIXED LEUKOCYTE CULTURE TEST: A PRELIMINARY REPORT
HISTOCOMPATIBILITY MATCHING VI. MINIATURIZATION OF THE MIXED LEUKOCYTE CULTURE TEST: A PRELIMINARY REPORT
复制标题
组织相容性匹配 VI．
作者：
Hartzman Rj;M. Segall;M. Bach;Fritz H. Bach
混合白细胞培养（MLC）测试的小型化方法描述，结果在细胞，培养基和时间的大量节省。测试在微滴板中进行，使用0.2 ml培养体积。反应细胞和刺激细胞的浓度因实验而异。当培养物从48小时开始标记16 - 20小时时，可以明显区分同基因和异基因混合物。该方法测量MLC身份，如前所述，并以与宏观方法显著相关的方式进行量化。
A miniaturized method for the mixed leukocyte culture (MLC) test is described which results in substantial savings in cells, medium, and time. The test is done in microtiter plates using 0.2-ml culture volumes. Concentrations of responding and stimulating cells are varied, depending on the experiment. Significant discrimination between isogeneic and allogeneic mixtures is possible when cultures are labeled for a 16–20-hr period starting at 48 hr. The method measures MLC identity, as previously defined, and quantitates in a manner significantly correlated with the macro method.