Interruption of TPA-induced signals by an antiviral and antitumoral xanthate compound: inhibition of a phospholipase C-type reaction.
Interruption of TPA-induced signals by an antiviral and antitumoral xanthate compound: inhibition of a phospholipase C-type reaction.
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抗病毒和抗肿瘤黄原酸盐化合物干扰 TPA 诱导的信号：抑制磷脂酶 C 型反应。
DOI：
10.1016/0006-291x(89)91981-5
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发表时间：
1989
影响因子：
3.1
通讯作者：
K. Müller
中科院分区：
文献类型：
--
作者：
K. Müller
研究了三环癸-9-基黄原酸酯对佛波酯TPA诱导的磷脂酰胆碱代谢变化的影响。在同时存在的黄原酸TPA未能刺激代谢[32 P]营业额的主要磷脂。在TPA D 609处理的细胞中，在脉冲标记后，前体分子[3 H]胆碱掺入磷脂酰胆碱中。因此，[32 P]磷脂酰胆碱营业额的减少似乎并不是由于TPA刺激的磷脂酰胆碱生物合成的抑制。然而，黄原酸酯对TPA刺激的[3 H]胆碱预标记细胞中[3 H]磷脂酰胆碱释放[3 H]磷酸胆碱产生抑制作用。此外，TPA诱导的二酰基甘油水平的升高在化合物的存在下降低。因此，这些结果提供了黄原酸盐抑制完整细胞中TPA诱导的磷脂酶C活性的证据。
The effect of tricyclodecan-9-yl-xanthogenate on the phorbolester TPA induced changes in phosphatidylcholine metabolism was investigated. In the simultaneous presence of the xanthate TPA failed to stimulate the metabolic [32 P] turnover of the major phospholipids. The precursor molecule [3 H] choline was incorporated into phosphatidylcholine after pulse labeling in TPA D609-treated cells. Thus, the reduction of the [32 P] phosphatidylcholine turnover did not appear to result from an inhibition of the TPA-stimulated phosphatidylcholine biosynthesis. However, the xanthate exerted an inhibitory effect on the TPA-stimulated liberation of [3 H] phosphorylcholine from [3 H] phosphatidylcholine in cells prelabeled with [3 H] choline. Furthermore, the TPA-induced rise in the diacylglycerol level was reduced in the presence of the compound. Thus, these results provide evidence that the xanthate inhibits a TPA-induced phospholipase C activity in the intact cell.
1,2-二酰基甘油和佛波酯刺激 GH3 垂体细胞中的磷脂酰胆碱代谢。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kolesnick,RN;Paley,AE
通讯作者：
Paley,AE