In vivo detection of optically-evoked opioid peptide release.
In vivo detection of optically-evoked opioid peptide release.
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DOI：
10.7554/elife.36520
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发表时间：
2018-09-03
期刊：
影响因子：
7.7
通讯作者：
Bruchas MR
中科院分区：
文献类型：
--
作者：
Al-Hasani R;Wong JT;Mabrouk OS;McCall JG;Schmitz GP;Porter-Stransky KA;Aragona BJ;Kennedy RT;Bruchas MR
尽管在过去的十年里，扰乱大脑神经回路的技术和技术有了加速的进步，但我们仍然缺乏足够的装备来充分剖析体内内源性多肽的释放。为此，我们开发了一种结合体内光遗传学和微透析的系统，以及基于高灵敏度的质谱学分析来测量自由活动的啮齿动物的阿片肽释放。
Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.