Characterization of OXA-181, a Carbapenem-Hydrolyzing Class D β-Lactamase from Klebsiella pneumoniae
Characterization of OXA-181, a Carbapenem-Hydrolyzing Class D β-Lactamase from Klebsiella pneumoniae
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DOI：
10.1128/aac.00481-11
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发表时间：
2011-10-01
影响因子：
4.9
通讯作者：
Poirel, Laurent
中科院分区：
文献类型：
--
作者：
Potron, Anaies;Nordmann, Patrice;Poirel, Laurent
肺炎克雷伯菌KP 3分离自从印度转移至阿曼苏丹国的患者。K. pneumoniae KP 3对所有β-内酰胺类抗生素（包括碳青霉烯类）具有耐药性，并表达碳青霉烯类水解β-内酰胺酶OXA-181，该酶与OXA-48的区别在于四个氨基酸取代。与OXA-48相比，OXA-181具有非常相似的水解特征。bla（OXA-181）基因位于7.6-kb ColE型质粒上，并与插入序列ISEcp 1连接。还证实了ISEcp 1介导的bla（OXA-181）的一端转座。
Klebsiella pneumoniae KP3 was isolated from a patient transferred from India to the Sultanate of Oman. K. pneumoniae KP3 was resistant to all beta-lactams, including carbapenems, and expressed the carbapenemhydrolyzing beta-lactamase OXA-181, which differs from OXA-48 by four amino acid substitutions. Compared to OXA-48, OXA-181 possessed a very similar hydrolytic profile. The bla(OXA-181) gene was located on a 7.6-kb ColE-type plasmid and was linked to the insertion sequence ISEcp1. The ISEcp1-mediated one-ended transposition of bla(OXA-181) was also demonstrated.