Expression and localization of two isoforms of AQP10 in human small intestine
Expression and localization of two isoforms of AQP10 in human small intestine
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DOI：
10.1042/bc20040091
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发表时间：
2005-11-01
影响因子：
2.7
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Li, HP;Kamiie, J;Yamamoto, T
背景资料。水通道蛋白10（aquaporin 10，AQP 10）是水通道的一个新成员。这些转录物之间的差异在于存在或不存在475 nt的插入，这导致翻译框移位，导致氨基酸翻译的不同终止和较短形式的AQP 10的合成。因此，从具有插入的转录物推导的AQP 10分子具有与从没有插入的转录物翻译的不同的C末端。在本研究中，我们研究了这些AQP 10亚型的表达和定位。两个转录本在人十二指肠和空肠中表达。使用这些AQP 10同种型的抗体的蛋白质印迹分析免疫印迹了两条约为100 μ g/ml的条带。分别为35和30 kDa。免疫组化和免疫电镜显示AQP 10定位于小肠绒毛毛细血管内皮细胞，而在胃肠道胰腺内分泌细胞中没有定位。AQP 10亚型的不同定位形式表明它们可能在人小肠近端部分发挥不同的作用。
Background information. AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino acid translation and synthesis of a shorter form of AQP10. Thus an AQP10 molecule deduced from the transcript with an insertion has a distinct C-terminus, different from that translated from the transcript without the insertion. In the present study, we investigated the expression and localization of these AQP10 isoforms.Results. Two transcripts were expressed in human duodenum and jejunum. Western-blot analysis using antibodies for these AQP10 isoforms immunoblotted two bands of approx. 35 and 30 kDa respectively. Immunolhistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro-entero-pancrestic endocrine cells.Conclusion. The different forms of localization of the AQP10 isoforms indicate that they may play different roles in the proximal portion of human small intestine.