A 3D-printed portable device for field deployment of living biosensors
A 3D-printed portable device for field deployment of living biosensors
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DOI：
10.1016/j.mex.2019.05.018
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发表时间：
2019-01-01
期刊：
影响因子：
1.9
通讯作者：
Long, Zhicheng
中科院分区：
文献类型：
--
作者：
Movizzo, Paul G.;Ruder, Warren C.;Long, Zhicheng
活体生物传感器通常使用转基因生物（GMO）来检测临床样本中的感染性生物标志物。许多法律禁止将转基因生物释放到环境中，这限制了活体生物传感器在实验室环境之外的应用。在这里，我们报告了一种强大的 3D 打印设备，可以消除潜在用户和环境中转基因生物暴露的风险。该装置设计用于紧密连接到普通培养管，由两个组件组成：外壳和柱塞。外壳包含一个应力集中切口和一个用于容纳活体生物传感器的试剂井。而柱塞设计为与外壳形成两级压配合密封。第一个密封允许将活体生物传感器从生物安全实验室安全转移到现场，第二个密封可防止转基因生物在测试期间和安全处置之前从培养管中泄漏。此外，还设计并 3D 打印了杠杆驱动机器，以帮助用户操作该设备。 (C) 2019 作者。由 Elsevier B.V. 出版
Living biosensors typically use genetically modified organisms (GMOs) to detect infectious biomarkers in clinical samples. GMOs are prohibited to be released into the environment by many laws, which limits the application of living biosensors outside laboratory settings. Here, we reported a robust 3D-printed device that eliminates the risk of exposure of GMOs to potential users and the environment. The device is designed to snugly attach to a common culture tube and consists of two components, a housing and a plunger. The housing contains a stress-focusing cutout and a reagent well to hold the living biosensor. While the plunger is designed to form a two-stage press-fit seal with the housing. The first seal allows to safely transfer the living biosensor from a biosafety lab to the field, and the second seal prevents the leakage of GMOs from the culture tube during the test and before safe disposal. Additionally, a lever-actuated machine was also designed and 3D-printed to assist users operating the device. (C) 2019 The Author(s). Published by Elsevier B.V.