The role of aligned polymer fiber-based constructs in the bridging of long peripheral nerve gaps
The role of aligned polymer fiber-based constructs in the bridging of long peripheral nerve gaps
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DOI：
10.1016/j.biomaterials.2008.03.042
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发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Bellamkonda, Ravi V.
中科院分区：
文献类型：
--
作者：
Kim, Young-tae;Haftel, Valerie K.;Bellamkonda, Ravi V.
跨越长神经间隙的周围神经再生在临床上具有挑战性。自体移植，治疗的标准，是有限的可用性和其他并发症。在这里，使用严格的解剖和功能的措施，我们报告说，对齐的聚合物纤维为基础的结构呈现地形线索，促进周围神经再生的长神经间隙。值得注意的是，对齐但不是随机取向的纤维引起再生，建立地形线索可以影响内源性神经修复机制，在没有外源性生长促进蛋白。轴突再生跨越17毫米的神经间隙，重新支配肌肉，并重新形成神经肌肉接头。电生理和行为分析表明，对齐，但不是随机取向的结构促进感觉和运动神经再生，显着改善功能的结果。此外，在对齐和随机取向的纤维膜上，体外DRG生长和体内神经再生的定量比较清楚地证明了亚微米尺度的地形线索在刺激内源性神经修复机制中的重要作用。(c)2008爱思唯尔有限公司保留所有权利。
Peripheral nerve regeneration across long nerve gaps is clinically challenging. Autografts, the standard of therapy, are limited by availability and other complications. Here, using rigorous anatomical and functional measures, we report that aligned polymer fiber-based constructs present topographical cues that facilitate the regeneration of peripheral nerves across long nerve gaps. Significantly, aligned but not randomly oriented fibers elicit regeneration, establishing that topographical cues can influence endogenous nerve repair mechanisms in the absence of exogenous growth promoting proteins. Axons regenerated across a 17 mm nerve gap, reinnervated muscles, and reformed neuromuscular junctions. Electrophysiological and behavioral analyses revealed that aligned but not randomly oriented constructs facilitated both sensory and motor nerve regeneration, significantly improved functional outcomes. Additionally, a quantitative comparison of DRG outgrowth in vitro and nerve regeneration in vivo on aligned and randomly oriented fiber films clearly demonstrated the significant role of sub-micron scale topographical cues in stimulating endogenous nerve repair mechanisms. (c) 2008 Elsevier Ltd. All rights reserved.