Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas
Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas
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DOI：
10.1016/j.marenvres.2008.02.010
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Bainy, Afonso C. D.
中科院分区：
文献类型：
--
作者：
de Toledo-Silva, Guilherme;Siebert, Marilia N.;Bainy, Afonso C. D.
We have cloned the full-length cDNA of the first member of a new cytochFOme P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331 bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500 bp and based on its deduced amino acid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A] is closely related to members of the CYPI 7 and CYP1 subfamilies. Semi-quantitative RT-PCR was performed to analyze the CYP356A] expression in different tissues ofthe oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356AI expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in xenobiotic biotransformation and/or steroid metabolism. (C) 2008 Elsevier Ltd. All rights reserved.
