ＨｙｐｅｒｔｈｅｒｍｉａにおけるＤＤＳの研究 特にｄｅｘｔｒａｎ ｍａｇｎｅｔｉｔｅ（ＤＭ）を用いるｉｎｄｕｃｔｉｖｅ ｈｅａｔｉｎｇについて:―特にdextran magnetite(DM)を用いるinductive heatingについて―
ＨｙｐｅｒｔｈｅｒｍｉａにおけるＤＤＳの研究 特にｄｅｘｔｒａｎ ｍａｇｎｅｔｉｔｅ（ＤＭ）を用いるｉｎｄｕｃｔｉｖｅ ｈｅａｔｉｎｇについて:―特にdextran magnetite(DM)を用いるinductive heatingについて―
复制标题
DDS 在热疗中的研究 特别是关于使用右旋糖酐磁铁矿 (DM) 的感应加热： - 特别是关于使用右旋糖酐磁铁矿 (DM) 的感应加热 -
DOI：
10.2745/dds.6.465
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发表时间：
1991
影响因子：
--
通讯作者：
Masao Fujimaki
中科院分区：
文献类型：
--
作者：
Shigeru Takemori;Kenji Tazawa;Hideo Nagae;I. Yamashita;H. Kato;Tokuzo Kasagi;Masato Maeda;Takashi Honda;Masao Fujimaki
“细胞内热疗”的概念是在施用产生磁激励的亚微米颗粒后癌细胞的局部感应加热，这些颗粒通过吞噬作用被摄入癌细胞内，导致癌细胞的选择性破坏，而对正常细胞和组织的影响很小。我们研究了细胞内高温对Meth-A，AH 60 C和VX-2肿瘤细胞的影响以及各种肿瘤细胞中DM的吞噬作用。葡聚糖磁铁矿被用作亚微米颗粒。DM颗粒不是葡聚糖和高铁酸盐的简单混合物，而是亚微米复合物。加热单元是具有产生电磁场的扁平型线圈的500 kHz的7 kW发生器。将DM颗粒注射到具有肿瘤细胞的动物中，腹膜内或瘤内注射。将这些动物暴露于温度超过43°C的外部感应场20分钟。用显微镜检查肿瘤细胞的吞噬作用。在给药后24小时，将DM颗粒细胞内摄取到肿瘤细胞中。以2次热疗组大鼠和小鼠的存活率及对VX-2肿瘤生长的抑制作用最明显。DM颗粒在500 kHz的感应加热中非常有前途。
The concept of “intracellular hyperthermia” is local inductive heating of cancer cells after administration of submicron particles producing magnetic excitation, These particles were taken in the cancer cells intracellularly by phagocytosis, resulting in selective destruction of cancer cells with little effect on normal cells and tissues. We examined effects of intracellular hyperthermia on Meth-A, AH60C and VX-2 tumor cells and a phagocytosis of DM in various tumor cells. Dextran magnetite was used as the submicron particle. DM particles are not simple mixture of dextran and ferrate but submicron complex. The heating unit was a 7 kW generator of 500 kHz with a pancake type coil creating electromagnetic field. DM particles were injected in animals with tumor cells intraperitoneally or intratumorously. These animals were exposed to the external inductive field for 20 min. at a temperature of more than 43°C. The phagocytosis of tumor cells was examined microscopically. DM particles were taken intracellularly in tumor cells at 24 hours after administration. The survival rate of rats and mice and the inhibition for growth of VX-2 tumor were most evident in the each groups of 2 times of hyperthermia. DM particles were very promising in inductive heating of 500 kHz.