REDUCTION OF THE CHLOROPLASTIC FRUCTOSE-1,6-BISPHOSPHATASE IN TRANSGENIC POTATO PLANTS IMPAIRS PHOTOSYNTHESIS AND PLANT-GROWTH
REDUCTION OF THE CHLOROPLASTIC FRUCTOSE-1,6-BISPHOSPHATASE IN TRANSGENIC POTATO PLANTS IMPAIRS PHOTOSYNTHESIS AND PLANT-GROWTH
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DOI：
10.1046/j.1365-313x.1994.6050637.x
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发表时间：
1994-11-01
期刊：
影响因子：
7.2
通讯作者：
WILLMITZER, L
中科院分区：
文献类型：
--
作者：
KOSSMANN, J;SONNEWALD, U;WILLMITZER, L
通过反义 RNA 技术创建了表达降低水平的果糖 1,6-双磷酸酶 (cp-FBPase) 叶绿体亚型的转基因马铃薯植物。选择具有不同水平FBPase活性的转化体并分析光合作用、碳代谢和生长。 FBPase 活性低于野生型水平的 15% 导致生长速度降低，这可能是由于光合活性降低所致。在 FBPase 活性低于野生型水平 15% 的植物中观察到块茎产量显着下降，而具有野生型酶活性 36% 的植物仍然具有正常的块茎产量，尽管它们表现出较低的光合能力。光合作用的减少也会导致叶子中总碳水化合物含量的减少。有趣的是，在表现出低于野生型 FBPase 活性 15% 的植物中观察到碳水化合物向可溶性糖的分配增加。当切下的叶盘在黑暗中放置在含蔗糖的培养基上时，源自 FBPase 活性降低的植物的叶盘会积累更多的淀粉。讨论了可能的影响。
Transgenic potato plants expressing reduced levels of the chloroplastic isoform of fructose-1,6-bisphosphatase (cp-FBPase) were created via the antisense RNA technique. Transformants with different levels of FBPase activity were selected and analysed with respect to photosynthesis, carbon metabolism, and growth. FBPase activity of less than 15% of wild-type levels led to reduced growth rates, probably due to the reduction of photosynthetic activity. A significant decrease in tuber yield is observed in plants with a FBPase activity below 15% of wild-type levels, whereas plants with 36% of wild-type enzyme activity still give normal tuber yields, even though they demonstrate a lowered photosynthetic capacity. Decreased photosynthesis also results in a reduction of total carbohydrate contents in leaves. Interestingly, increased carbohydrate partitioning towards soluble sugars is observed in plants displaying less than 15% of the wild-type FBPase activity. When excised leaf discs are placed on sucrose-containing media in darkness, discs derived from plants with a reduced FBPase activity accumulate higher amounts of starch. Possible implications are discussed.