Isolation and characterization of a rat luteal cDNA encoding 20 alpha-hydroxysteroid dehydrogenase.
Isolation and characterization of a rat luteal cDNA encoding 20 alpha-hydroxysteroid dehydrogenase.
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编码 20 α-羟基类固醇脱氢酶的大鼠黄体 cDNA 的分离和表征。
DOI：
10.1006/bbrc.1994.1844
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发表时间：
1994
影响因子：
3.1
通讯作者：
Gibori,G
中科院分区：
文献类型：
--
作者：
Mao,J;Duan,WR;Albarracin,CT;Parmer,TG;Gibori,G
我们报道了大鼠黄体20α羟基类固醇脱氢酶全长基因的分离和鉴定。20αHSD克隆编码的蛋白质氨基酸序列由323个氨基酸组成，分子量约为37 kDa。在开放阅读框的翻译序列中发现了来自纯化蛋白的多肽序列。基因序列和氨基酸序列分析表明，大鼠卵巢20α脱氢酶属于醛酮还原酶家族。Northern分析显示，黄体溶解后的黄体组织中有1.2kb的20α的HSD基因。催乳素显著降低20αHSDmRNA的表达。在被检测的其他组织中没有检测到信号，表明该酶在黄体中特异表达。
We report the isolation and characterization of a full length cDNA encoding rat 20αhydroxysteroid dehydrogenase derived from rat corpus luteum RNA. The predicted amino acid sequence of the protein encoded by the 20αHSD clone is composed of 323 amino acids possessing an approximate molecular weight of 37 kDa. The sequence of peptides derived from the purified protein was found in the translated sequence of the open reading frame. cDNA and amino acid sequence indicate that the rat ovarian 20αHSD belongs to the aldo-keto reductase family of enzymes. Northern analysis revealed a 1.2 Kb 20αHSD mRNA in corpora lutea undergoing luteolysis. Prolactin reduced markedly 20αHSD mRNA expression. No signal was detected in other tissues examined, demonstrating the specific expression of this enzyme in the corpus luteum.