Effects of Endurance Training on Three Superoxide Dismutase Isoenzymes in Human Plasma
Effects of Endurance Training on Three Superoxide Dismutase Isoenzymes in Human Plasma
复制标题
DOI：
10.1080/1071576031000102132
复制
发表时间：
2003-07
影响因子：
3.3
通讯作者：
T. Ookawara;S. Haga;S. Ha;S. Oh-Ishi;K. Toshinai;T. Kizaki;L. Ji;Keiichiro Suzuki;H. Ohno
中科院分区：
文献类型：
--
作者：
T. Ookawara;S. Haga;S. Ha;S. Oh-Ishi;K. Toshinai;T. Kizaki;L. Ji;Keiichiro Suzuki;H. Ohno
研究了耐力训练和急性力竭运动对血浆三种超氧化物歧化酶(SOD)同工酶水平和中性粒细胞产生超氧化物歧化酶能力的影响。18名年龄在17-22岁的健康男性学生自愿参加了为期三个月的游泳或跑步耐力训练。在训练课前后分别进行急性运动，并在运动前后采集血样。耐力训练显著提高所有受试者的最大摄氧量(VO2max)。耐力训练和急性运动对血浆铜锌超氧化物歧化酶水平均无影响。急性运动训练后，但不是在训练前，血浆锰-超氧化物歧化酶和细胞外超氧化物歧化酶(EC-SOD)水平分别提高了33.6%和33.5%。训练使安静时EC-SOD水平下降22.2%。急性运动在训练后，但不是在训练之前，增加了血浆过氧化脂质水平，这表明训练对象在力竭运动中有更高的氧化应激。急性运动提高了中性粒细胞产生超氧化物的能力，但训练后抑制了对超氧化物的诱导。这些结果表明，EC-SOD的变化方式不同于CuZn-SOD和Mn-SOD：训练使EC-SOD降低，而急性运动使EC-SOD升高，提示耐力训练增加了组织中EC-SOD的储备。提示血浆EC-SOD测定作为耐力训练新指标的可能性。
The effects of endurance training and acute exhaustive exercise on plasma levels of three superoxide dismutase (SOD) isoenzymes and the ability of superoxide generation in neutrophils were studied. Eighteen healthy male students, aged 17–22 years, who volunteered for this study, underwent three months of endurance training in swimming or running. Before and after the training course, they performed acute exercise and blood samples were collected before and after this exercise. The endurance training significantly increased maximal oxygen uptake (V˙O2max) in all subjects. Neither the endurance training nor the acute exercise affected the plasma CuZn-SOD level. Acute exercise after the training, but not before the training, increased both the plasma Mn-SOD and extracellular SOD (EC-SOD) levels by 33.6 and 33.5%, respectively. The training decreased the EC-SOD level at rest by 22.2%. Acute exercise after the training, but not before the training, increased the plasma lipid peroxide level, suggesting higher oxidative stress in trained subjects during exhaustive exercise. The ability of neutrophils to generate superoxide was increased by the acute exercise, but induction of the superoxide was suppressed after training. These results indicate that EC-SOD levels were changed in a different manner from the CuZn-SOD and Mn-SOD: it was decreased by training but was increased by acute exercise, suggesting that endurance training increases the reserve of EC-SOD in tissues. The results also suggest the possibility of plasma EC-SOD assay as a new index of endurance training.