RECOMBINANT TYPE-5 ADENOVIRUSES EXPRESSING BOVINE PARAINFLUENZA VIRUS TYPE-3 GLYCOPROTEINS PROTECT SIGMODON-HISPIDUS COTTON RATS FROM BOVINE PARAINFLUENZA VIRUS TYPE-3 INFECTION
RECOMBINANT TYPE-5 ADENOVIRUSES EXPRESSING BOVINE PARAINFLUENZA VIRUS TYPE-3 GLYCOPROTEINS PROTECT SIGMODON-HISPIDUS COTTON RATS FROM BOVINE PARAINFLUENZA VIRUS TYPE-3 INFECTION
复制标题
DOI：
10.1128/jvi.69.7.4308-4315.1995
复制
发表时间：
1995-07-01
影响因子：
5.4
通讯作者：
BABIUK, LA
中科院分区：
文献类型：
--
作者：
BREKERKLASSEN, MM;YOO, DW;BABIUK, LA
使用棉鼠来研究 3 型牛副流感病毒 (bPIV3) 的复制和发病机制，并测试 F 和 HN 糖蛋白在调节感染中的功效。病毒回收、免疫荧光、免疫沉淀和合胞体诱导表明，棉鼠肺细胞的体外培养物支持 bPIV3 的生长。棉鼠鼻内 (i.n.) 接种 10(7) PFU，导致病毒在 2 天后达到峰值恢复（8 x 10(4) PFU/g 肺组织），并在感染后 5 至 7 天出现明显的细支气管炎和淋巴细胞浸润。肺部和气管的免疫组织化学染色表明，病毒抗原阳性细胞的频率在感染过程中增加，在第 5 天达到最大值。通过酶联免疫吸附测定 (ELISA)、血凝抑制 (HAI) 和血清中和 (SN) 评估血清抗体反应。跟随单个 in.n.接种后，ELISA、HAI、SN检测血清抗体水平分别为1/40、960、1/32、1/80。当在注射前 10 天进行 10(7) PFU 肌肉注射时。接种，诱导产生1/163,000的ELISA滴度、1/640的HAI滴度和1/512的SN滴度的二次反应，含有bPIV3F或HN蛋白或两种病毒的组合的重组腺病毒5型的IN接种保护棉鼠免受bPIV3攻击，通过ELISA、HAI和SN滴度对保护作用进行血清学评估，组织病理学、免疫组织化学和病毒回收。
Cotton rats were used to study the replication and pathogenesis of bovine parainfluenza virus type 3 (bPIV3) and to test the efficacy of the F and HN glycoproteins in modulating infection. In vitro cultures of cotton rat lung cells supported the growth of bPIV3 as shown by virus recovery, immunofluorescence, immunoprecipitation, and syncytium induction. Intranasal (i.n.) inoculation of cotton rats with 10(7) PFU resulted in peak recovery of virus after 2 days (8 x 10(4) PFU/g of lung tissue) and significant bronchiolitis with lymphocyte infiltration 5 to 7 days postinfection. Immunohistochemical staining of lungs and trachea demonstrated that virus antigen-positive cells increased in frequency over the course of infection to a maximum on day 5. Serum antibody responses were evaluated by enzyme-linked immunosorbent assays (ELISA), hemagglutination inhibition (HAI), and serum neutralization (SN). Following a single i.n. inoculation, serum antibody levels were 1/40,960, 1/32, and 1/80, as detected by ELISA, HAI, and SN, respectively. When an intramuscular inoculation of 10(7) PFU was administered 10 days prior to the i.n. inoculation, a secondary response which resulted in an ELISA titer of 1/163,000, an HAI titer of 1/640, and an SN titer of 1/512 was induced, IN inoculation of recombinant adenoviruses type 5 containing the bPIV3 F or HN protein or a combination of the two viruses protected cotton rats from bPIV3 challenge, Protection was evaluated serologically by ELISA, HAI, and SN titers, histopathology, immunohistochemistry, and virus recovery.