Nonrandom Germline Transmission of Mouse spermatogonial Stem Cells
Nonrandom Germline Transmission of Mouse spermatogonial Stem Cells
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DOI：
10.1016/j.devcel.2016.07.011
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发表时间：
2016-08-08
影响因子：
11.8
通讯作者：
Shinohara, Takashi
中科院分区：
文献类型：
--
作者：
Kanatsu-Shinohara, Mito;Naoki, Honda;Shinohara, Takashi
基因被认为是通过少量卵母细胞与大量精子的随机受精传递给后代的。在这里，我们分析了动态的男性生殖传递的遗传标记和精原干细胞（SSCs）的移植。我们发现，来自少数特定SSC的后代在有限的时间内出现。有趣的是，相同的SSC克隆稍后再次出现，平均功能寿命接近124.4天。周期性的后代生产从SSC中没有引起SSC自我更新活动的变化，因为谱系追踪分析表明，所有的SSC积极增殖。选择似乎发生在精原细胞分化阶段，当广泛的细胞凋亡观察。生殖系传播的模式可以用一个数学模型来预测，在这个模型中，精原干细胞重复短暂的生精爆发和不应期的循环。因此，精子发生是一个受调控的过程，其中特定的SSC克隆被反复招募用于长期周期的受精。
Genes are thought to be transmitted to offspring by random fertilization of a small number of oocytes with numerous spermatozoa. Here we analyzed the dynamics of male germline transmission by genetic marking and transplantation of spermatogonial stem cells (SSCs). We found that offspring deriving from a small number of specific SSCs appear within a limited time. Interestingly, the same SSC clones reappear later with an average functional lifespan of similar to 124.4 days. Cyclic offspring production from SSCs was not caused by changes in SSC self renewal activity because lineage-tracing analyses suggested that all SSCs actively proliferated. Selection appears to occur during the differentiating spermatogonia stage, when extensive apoptosis was observed. The pattern of germline transmission could be predicted using a mathematical model in which SSCs repeat cycles of transient spermatogenic burst and refractory periods. Thus, spermatogenesis is a regulated process whereby specific SSC clones are repeatedly recruited for fertilization with long-term cycles.