Normal mouse kidneys contain activated and CD3+CD4-CD8- double-negative T lymphocytes with a distinct TCR repertoire
Normal mouse kidneys contain activated and CD3+CD4-CD8- double-negative T lymphocytes with a distinct TCR repertoire
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DOI：
10.1189/jlb.0907651
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发表时间：
2008-12-01
影响因子：
5.5
通讯作者：
Rabb, Hamid
中科院分区：
文献类型：
--
作者：
Ascon, Dolores B.;Ascon, Miguel;Rabb, Hamid
健康的肝脏、肠、肺和皮肤都有常规和非常规表型的常驻淋巴细胞。在健康小鼠肾脏中也检测到淋巴细胞；然而，这些细胞还没有得到很好的研究，而且在很大程度上被忽视了。为了更好地表征肾内淋巴细胞，我们用PBS广泛灌注C57BL/6J小鼠，然后分离单个核细胞进行流式细胞术分析。我们观察了大量灌注后正常小鼠肾脏中的T细胞、B细胞和NK细胞。大约50%的肾T淋巴细胞表达中等水平的CD3（CD3(int) T细胞）。与肝和肺相似，在正常小鼠肾脏中观察到高比例的非常规CD3(+)CD4(-)CD8（-）双阴性T细胞，其中11%表达B220抗原。与脾脏和血液不同，肾脏中的经典CD4（+）和CD8(+) T淋巴细胞具有高比例的活化CD69（+）和效应/记忆CD44CD62L配体表型。此外，在灌注和渗出的肾脏中观察到少量CD4（+）、CD25（+）叉头盒p3（+）和NKT细胞。此外，与来自脾脏的T细胞相比，在肾内常规和非常规T细胞上发现了不同的TCR库。最后，在肾缺血再灌注损伤（IRI） 24小时后，观察到从灌注肾中分离的CD4（+）和CD8(+) T细胞产生的细胞因子ifn - γ和tnf - α增加。这些数据表明，这些细胞中的一些窝藏在肾脏可能涉及IRI致病过程的免疫反应。j . Leukoc。生物学报。84:1400-1409；2008.
Healthy liver, intestine, lung, and skin harbor resident lymphocytes with conventional and unconventional phenotypes. Lymphocytes also have been detected in healthy mice kidneys; however, these cells have not been well studied and have been largely overlooked. To better characterize the intra-renal lymphocytes, we extensively perfused C57BL/6J mice with PBS and then isolated mononuclear cells for flow cytometry analysis. We observed T cells, B cells, and NK cells in normal mice kidneys after extensive perfusion. Approximately 50% of kidney T lymphocytes expressed intermediate levels of CD3 (CD3(int) T cells). Similar to liver and lung, a high percentage of unconventional CD3(+)CD4(-)CD8(-) double-negative T cells was observed in normal mice kidneys, from which 11% expressed B220 antigen. Unlike the spleen and blood, the classic CD4(+) and CD8(+) T lymphocytes in the kidney had a high proportion of activated CD69(+) and effector/memory CD44CD62L ligand phenotypes. Also, a small percentage of CD4(+)CD25(+) forkhead box p3(+) and NKT cells was observed in perfused and exanguinated kidneys. In addition, a distinct TCR repertoire was found on intra-renal conventional and unconventional T cells compared with those from the spleen. Finally, after 24 h of renal ischemia reperfusion injury (IRI), increased production of cytokines IFN-gamma and TNF-alpha by CD4(+) and CD8(+) T cells, isolated from perfused kidneys, was observed. These data suggest that some of these cells harbored in the kidney could be implicated in the immune response of the IRI pathogenic process. J. Leukoc. Biol. 84: 1400-1409; 2008.