Coenzyme A and its thioester pools in fasted and fed rat tissues
Coenzyme A and its thioester pools in fasted and fed rat tissues
复制标题
禁食和进食大鼠组织中的辅酶 A 及其硫酯库
DOI：
10.1016/j.bbrc.2010.10.009
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发表时间：
2010
影响因子：
3.1
通讯作者：
Chohnan S
中科院分区：
文献类型：
--
作者：
Tokutake Y;Onizawa N;Katoh H;Toyoda A;Chohnan S
用酰基辅酶A(CoA)循环法分析了禁食和喂养大鼠组织中3种辅酶A(CoA)分子的水平，即非酯化CoA(CoASH)、乙酰辅酶A和丙二酰辅酶A。脑组织中的丙二酰辅酶A随饲料的增加而增加，反之，乙酰辅酶A随饲料的增加而减少。这种现象发生在大脑皮层、海马体、小脑和延髓，以及通过监测丙二酰辅酶A来控制能量平衡的下丘脑。在非脑组织中，乙酰辅酶A、丙二酰辅酶A和Coash池的大小取决于组织。与包括脑组织在内的其他组织相比，肝脏、心脏和棕色脂肪组织中由上述三种辅酶A组成的CoA总库较大，而肾周、附睾部和卵巢脂肪组织的CoA总库较小。此外，每个辅酶A池对摄食的反应并不一致，这表明组织特异性代谢在非脑组织中单独发挥作用。因此，对13种大鼠组织的综合分析显示，CoA池有不同的大小，并根据组织的不同对禁食和进食表现出不同的反应。
Levels of three coenzyme A (CoA) molecular species, i.e., nonesterified CoA (CoASH), acetyl-CoA, and malonyl-CoA, in fasted and fed rat tissues were analyzed by the acyl-CoA cycling method which makes detection possible at the pmol level. Malonyl-CoA in brain tissues readily increased with feeding, and inversely, acetyl-CoA decreased. This phenomenon occurred in the cerebral cortex, hippocampus, cerebellum, and medulla oblongata, as well as in the hypothalamus which controls energy balance by monitoring malonyl-CoA. In the non-brain tissues, the sizes of the acetyl-CoA, malonyl-CoA, and CoASH pools depended on the tissues. The total CoA pools consisting of the above three CoA species in the liver, heart, and brown adipose tissue were larger and those of the perirenal, epididymal, and ovarian adipose tissues were much smaller, compared with those of other tissues including brain tissues. In addition, the response of each CoA pool to feeding was not uniform, suggesting that the tissue-specific metabolism individually functions in the non-brain tissues. Thus, a comprehensive analysis of thirteen types of rat tissue revealed that CoA pools have different sizes and showed a different response to fasting and feeding depending on the tissue.