Renal iodide clearance in rabbits.
Renal iodide clearance in rabbits.
复制标题
兔子肾脏碘化物清除率。
DOI：
10.1530/acta.0.1210246
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
S. Vadstrup
中科院分区：
文献类型：
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作者：
S. Vadstrup
本研究的目的是比较基于血浆值的间接清除方法和使用皮肤附着的小型放射性探测器的外部检测活性与基于持续输注和尿液收集的直接清除方法。实验在麻醉的家兔身上进行。血浆碘浓度增加100倍，以防止放射性碘的甲状腺组织。[131I]碘以恒定速率输注，[125I]碘以静脉给药间接清除率测定。在所有实验中，血浆125I活性值与外部125I活性值的比值从施用[125I]碘剂后15分钟开始直至整个实验过程中都是恒定的。3只家兔7次实验结果表明，在直接廓清值（0.002 ~ 0.6 ml.min-1.kg-1）范围内，直接廓清值与间接廓清值呈极显著相关（r = 0.98）。直接间隙和间接间隙在2 h稳态期间同时测量。直接清除率和间接清除率平均比值的95%置信区间为血浆活度+/- 20%，外部测量活度+/- 26%。结果表明，两种间接清除率测定方法均可用于家兔肾脏碘化物清除率的测定。
The purpose of the study was to compare indirect clearance methods based on plasma values and external detection of activity using small skin attached radioactivity detectors with a direct clearance method based on constant infusion and urine collection. The experiments were performed in anesthetized rabbits. The plasma iodide concentration was increased 100 times to prevent thyroid organification of radioactive iodide. [131I]iodide was infused at a constant rate and [125I]iodide was administered iv as a bolus for indirect clearance determination. The ratio between plasma values and external values 125I-activity was constant in all experiments from 15 min after administration of [125I]iodide and throughout the experiment. The results of 7 experiments in 3 rabbits showed a highly significant correlation (r = 0.98) between direct and indirect values within the range of direct clearance values obtained (0.002-0.6 ml.min-1.kg-1. The direct and indirect clearances were measured simultaneously during a 2-h period of steady state. The 95% confidence interval of the mean ratio between direct and indirect clearances was +/- 20% for plasma activity and +/- 26% for externally measured activity. It is concluded that both methods of indirect clearance determination can be applied to rabbits to estimate absolute values of the renal iodide clearance.