Mapping QTL for grain yield, yield components, and spike features in a doubled haploid population of bread wheat
Mapping QTL for grain yield, yield components, and spike features in a doubled haploid population of bread wheat
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DOI：
10.1139/g11-017
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发表时间：
2011-06-01
期刊：
影响因子：
3.1
通讯作者：
Suenaga, Kazuhiro
中科院分区：
文献类型：
--
作者：
Heidari, Bahram;Sayed-Tabatabaei, Badraldin Ebrahim;Suenaga, Kazuhiro
来自日本品种“Fukuho-kumogi”和以色列小麦品系“Oligoculm”杂交的双单倍体 (DH) 群体被用来绘制与小麦 (Triticum aestivum L) 谷物产量、产量组成和穗特征表达相关的基因组区域。总共使用371个标记（RAPD、SSR、RFLP、AFLP和两个形态性状）构建了覆盖4190 cM小麦基因组的连锁图谱，包括28个连锁群。所有研究性状的复合区间作图结果表明，一些数量性状基因座（QTL）在2004年和2005年进行的实验中保持稳定。位于1A染色体Hair-Xpsp2999区间的主要QTL控制籽粒/穗的表达（R-2 = 2004年的12.9%和2005年的22.4%）、粒重/穗的表达（R-2 = 2004 年为 21.4%，2005 年为 15.8%），以及峰值数量（R-2 = 2004 年为 15.6%，2005 年为 5.4%）。 2004年和2005年，位于6A、6B和6D染色体上的籽粒产量QTL分别占该性状总变异的27.2%和31.7%。从“Oligoculm”继承的等位基因增加了穗的长度，并且对穗数量的影响减少。根据2005年获得的数据，基因座Xgwm261与高度显着的穗长QTL（R-2 = 42.33%）相关，也是小穗紧密度的主要QTL（R-2 = 26.1%）。
A doubled haploid (DH) population derived from a cross between the Japanese cultivar 'Fukuho-kumogi' and the Israeli wheat line 'Oligoculm' was used to map genome regions involved in the expression of grain yield, yield components, and spike features in wheat (Triticum aestivum L). A total of 371 markers (RAPD, SSR, RFLP, AFLP, and two morphological traits) were used to construct the linkage map that covered 4190 cM of wheat genome including 28 linkage groups. The results of composite interval mapping for all studied traits showed that some of the quantitative trait loci (QTL) were stable over experiments conducted in 2004 and 2005. The major QTL located in the Hair-Xpsp2999 interval on chromosome 1A controlled the expression of grains/spike (R-2 = 12.9% in 2004 and 22.4% in 2005), grain weight/spike (R-2 = 21.4% in 2004 and 15.8% in 2005), and spike number (R-2 = 15.6% in 2004 and 5.4% in 2005). The QTL for grain yield located on chromosomes 6A, 6B, and 6D totally accounted for 27.2% and 31.7% of total variation in this trait in 2004 and 2005, respectively. Alleles inherited from 'Oligoculm' increased the length of spikes and had decreasing effects on spike number. According to the data obtained in 2005, locus Xgwm261 was associated with a highly significant spike length QTL (R-2 = 42.33%) and also the major QTL for spikelet compactness (R-2 = 26.1%).