Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease.
Gene expression profiles in a panel of childhood leukemia cell lines mirror critical features of the disease.
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发表时间：
2003-07
影响因子：
5.7
通讯作者：
U. Kees;J. Ford;Marcia Watson;A. Murch;M. Ringnér;R. Walker;P. Meltzer
中科院分区：
文献类型：
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作者：
U. Kees;J. Ford;Marcia Watson;A. Murch;M. Ringnér;R. Walker;P. Meltzer
抗癌新药的开发需要建立细胞系。它们需要用于体外研究以鉴定候选药物，以及用于异种移植模型以测量体内药物功效。用于评价潜在新型治疗剂的细胞系需要满足特定标准。它们必须显示所研究的特定癌症的特征。考虑到癌症的异质性，需要代表相关的亚型。在这项研究中，我们已经检查了这些方面的儿童急性淋巴细胞白血病。对本实验室最近建立的一组13个白血病细胞系进行了分析。我们使用cDNA微阵列来定义基因表达谱，并将数据与免疫表型和细胞遗传学分析进行比较。所获得的表达谱与相应的蛋白质水平显示出极好的一致性。重要的是，这组细胞系显示了在儿童白血病患者中临床重要的急性淋巴细胞白血病亚型中鉴定的关键遗传特征。
The development of new drugs against cancer requires established cell lines. They are needed for in vitro studies to identify candidate drugs and in xenograft models to measure drug efficacy in vivo. Specific criteria need to be fulfilled by cell lines used in the evaluation of potential novel therapeutic agents. It is imperative that they display the features of the particular cancer under investigation. Given the documented heterogeneity of cancers, relevant subtypes need to be represented. In this study, we have examined these aspects for pediatric acute lymphoblastic leukemia. A panel of 13 leukemia cell lines recently established in our laboratory was analyzed. We used cDNA microarrays to define the gene expression profiles and compared the data with immunophenotyping and cytogenetic analyses. The expression profiles obtained showed excellent concordance with corresponding protein levels. Importantly, the panel of lines displayed the critical genetic features identified in clinically important acute lymphoblastic leukemia subtypes in childhood leukemia patients.