Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function
Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function
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DOI：
10.1152/japplphysiol.00791.2006
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发表时间：
2007-03-01
影响因子：
3.3
通讯作者：
Nair, K. Sreekumaran
中科院分区：
文献类型：
--
作者：
Chow, Lisa S.;Greenlund, Laura J.;Nair, K. Sreekumaran
我们假设有氧运动训练后骨骼肌线粒体功能增强与线粒体转录因子、DNA丰度[线粒体DNA（mtDNA）]和线粒体相关基因转录水平以及自发性体力活动（SPA）水平的增加有关。我们报告了每天跑步机训练对12周龄FVB小鼠的影响，在80%的峰值O-2消耗量下进行5天/周超过8周，并研究了训练对身体组成，葡萄糖耐量，肌肉mtDNA，肌肉，线粒体相关基因转录，体外肌肉线粒体ATP生产能力（MATPC）和SPA水平变化的影响。与未训练的小鼠相比，训练的小鼠具有更高的峰值O2消耗量（+ 18%; P < 0.001），腹部百分比较低（-25.4%; P < 0.02）和体脂（-19.5%; P < 0.01），改善糖耐量（P < 0.04），肌肉线粒体酶活性（+19.5-43.8%; P < 0.04）和MATPC（+28.9%至+32.4%; P < 0.01）较高。基因阵列分析显示，训练和未训练的小鼠之间的神经元相关本体组的mRNA存在显着差异。训练还增加了肌肉线粒体DNA（+88.4至+110%; P < 0.05）、过氧化物酶体增殖激活受体-γ共激活因子-1 α蛋白（+99.5%; P < 0.04）和线粒体转录因子A mRNA水平（+21.7%; P < 0.004）。SPA水平在训练的小鼠中更高（P = 0.056，双侧t检验），并且与两个单独的基于基质的MATPC测量值显著相关（P < 0.02）。总之，有氧运动训练增强了肌肉线粒体转录因子、mtDNA丰度、线粒体相关基因转录水平和线粒体功能，并且这种线粒体功能的增强与SPA增加相关。
We hypothesized that enhanced skeletal muscle mitochondrial function following aerobic exercise training is related to an increase in mitochondrial transcription factors, DNA abundance [ mitochondrial DNA ( mtDNA)], and mitochondria-related gene transcript levels, as well as spontaneous physical activity ( SPA) levels. We report the effects of daily treadmill training on 12-wk-old FVB mice for 5 days/wk over 8 wk at 80% peak O-2 consumption and studied the training effect on changes in body composition, glucose tolerance, muscle mtDNA muscle, mitochondria-related gene transcripts, in vitro muscle mitochondrial ATP production capacity (MATPC), and SPA levels. Compared with the untrained mice, the trained mice had higher peak O2 consumption (+ 18%; P < 0.001), lower percentage of abdominal (-25.4%; P < 0.02) and body fat (-19.5%; P < 0.01), improved glucose tolerance (P < 0.04), and higher muscle mitochondrial enzyme activity (+19.5 - 43.8%; P < 0.04) and MATPC (+28.9 to +32.4%; P < 0.01). Gene array analysis showed significant differences in mRNAs of mitochondria-related ontology groups between the trained and untrained mice. Training also increased muscle mtDNA (+88.4 to +110%; P < 0.05), peroxisome proliferative-activated receptor-gamma coactivator-1 alpha protein (+99.5%; P < 0.04), and mitochondrial transcription factor A mRNA levels (+21.7%; P < 0.004) levels. SPA levels were higher in trained mice (P = 0.056, two-sided t-test) and significantly correlated with two separate substrate-based measurements of MATPC (P < 0.02). In conclusion, aerobic exercise training enhances muscle mitochondrial transcription factors, mtDNA abundance, mitochondria-related gene transcript levels, and mitochondrial function, and this enhancement in mitochondrial function occurs in association with increased SPA.