Growth of Vibrio anguillarum in salmon intestinal mucus
Growth of Vibrio anguillarum in salmon intestinal mucus
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DOI：
10.1128/aem.63.3.1034-1039.1997
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发表时间：
1997-03-01
影响因子：
4.4
通讯作者：
Nelson, DR
中科院分区：
文献类型：
--
作者：
Garcia, T;Otto, K;Nelson, DR
研究了鳗弧菌在鲑鱼肠黏液中生长的生理变化，比较了添加黏液培养基和不添加黏液培养基中生长和饥饿时鳗弧菌的生长、存活和蛋白表达变化。在以黏液为唯一碳源生长后，鳗鲡的CFU迅速下降。在2% NaCl浓度的Luria肉汤、低葡萄糖肉汤（3M）或饥饿期间无碳、无氮、无磷盐溶液（NSS）中均未观察到这种下降。用[S-35]蛋氨酸标记细胞，并通过一、二维凝胶电泳和放射自显影分析标记蛋白，检测黏液生长过程中蛋白表达的变化。从3m培养的细胞和nss饥饿细胞中比较[S-35]蛋氨酸标记的蛋白，发现了四种新生黏液诱导蛋白（Mips）。这些Mips定位于鳗的膜部分。此外，至少有一种其他膜蛋白被发现在黏液生长的反应中表达增加。
The physiological changes of Vibrio anguillarum in response to growth in salmon intestinal mucus were investigated, Growth, survival, and changes in protein expression during growth in media supplemented with mucus were compared to growth and starvation in the identical media without mucus. V. anguillarum exhibited a rapid decline in CFU following growth in mucus as the sole carbon source. No such decline was observed in Luria broth with a 2% NaCl concentration, in glucose-minimal broth (3M), or during starvation in a carbon-, nitrogen-, and phosphorus-free salt solution (NSS). The changes in protein expression during growth in mucus were examined by labeling cells with [S-35]methionine and analyzing the labeled proteins by one- and two-dimensional gel electrophoresis and autoradiography. Comparison of [S-35] methionine-labeled proteins from cells with 3M-grown cells and NSS-starved cells revealed four de novo mucus-inducible proteins (Mips). These Mips were localized in the membrane fraction of V. anguillarum. Additionally, at least one other membrane protein was found to have increased expression in response to growth in mucus.