Type I and type IV collagen promote adherence and spreading of human type II pneumocytes in vitro.
Type I and type IV collagen promote adherence and spreading of human type II pneumocytes in vitro.
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I 型和 IV 型胶原蛋白在体外促进人 II 型肺细胞的粘附和扩散。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
H. Müller
中科院分区：
文献类型：
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作者：
T. Papadopoulos;L. Ionescu;J. Dämmrich;H. Toomes;H. Müller
人肺组织用DISPASE酶消化，然后在不连续的甲硝唑胺梯度上分离II型肺泡细胞。在新鲜分离细胞的原代黏附实验中，48h后I型胶原(CI)、IV型胶原(CIV)、纤维连接蛋白(FN)、层粘连蛋白(LM)和组织培养塑料的平均黏附率分别为21.7%、19.6%、13.4%、6.8%和7.0%。其次，对预先在CI、FN、小鼠EHS细胞基质和培养塑料上培养的II型肺泡细胞进行再粘连和铺展试验。培养48~72h后，分别在CI、CIV、FN、LM、CI+FN、CI+LM、CIV+FN、CIV+Lm基质上复制细胞，观察预培养基质对这些细胞的黏附反应是否有调节作用。复吸后1小时的再粘附值是48小时后初次粘附值的3.3-8.6倍。在纤维连接蛋白上预培养后，在含有胶原的基质上获得最高的再粘附值。在含有胶原的基质上重复培养后的铺展值在35.4%~6.6%之间，在FN上预培养后再次达到最高值。只有不到6.9%和0.5%的细胞在纯FN和纯LM上生长。目前的研究数据表明，人类II型肺泡细胞优先黏附和扩散在胶原基质上，而不是细胞外基质的其他成分上。这可能有助于我们了解这些细胞在体内的功能活动，特别是关于受损肺泡的再上皮化。
Human lung tissue was enzymatically digested with dispase and type II pneumocytes were subsequently separated on a discontinuous metrizamide gradient. In primary adherence assays with freshly isolated cells, mean adherence values of 21.7, 19.6, 13.4, 6.8 and 7.0% were obtained after 48 hours on type I collagen (CI), type IV collagen (CIV), fibronectin (FN), laminin (LM) and tissue culture plastic, respectively. Secondly, readherence and spreading assays were performed with type II pneumocytes, previously cultured on CI, FN, matrix of murine EHS cells, and culture plastic. After a culture period of 48 to 72 hours, the cells were replated and tested on CI, CIV, FN, LM, CI + FN, CI + LM, CIV + FN and CIV + LM substrata in order to find out whether preculture substrata can modulate the adherence response of these cells. Readherence values, 1 hour after replating, were 3.3- to 8.6-fold higher than primary adherence values after 48 hours. The highest readherence values were always obtained on collagen-containing substrata after preculture on fibronectin. Spreading values after replating ranged between 35.4 and 6.6% on collagen-containing substrata, the highest values were again obtained after preculture on FN. Less than 6.9% and 0.5% of the cells spread on pure FN and pure LM, respectively. The data of the present investigation indicate that human type II pneumocytes adhere and spread preferentially on collagenous substrata rather than on other components of the extracellular matrix. This might be important in helping us to understand the functional in vivo activity of these cells, especially as regards re-epithelialization of the injured pulmonary alveolus.