A betabaculovirus encoding a gp64 homolog.
A betabaculovirus encoding a gp64 homolog.
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DOI：
10.1186/s12864-016-2408-9
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发表时间：
2016-02-04
期刊：
影响因子：
4.4
通讯作者：
Wolff JL
中科院分区：
文献类型：
--
作者：
Ardisson-Araújo DM;Pereira BT;Melo FL;Ribeiro BM;Báo SN;de A Zanotto PM;Moscardi F;Kitajima EW;Sosa-Gomez DR;Wolff JL
从巴西甘蔗和其他单子叶植物栽培的最重要的害虫之一--甘蔗叶螟（Diatraea saccharalis）（鳞翅目：草螟科（Crambidae））中分离到一种甜菜杆状病毒（DisaGV）。使用454-焦磷酸测序法测定DisaGV的全基因组序列。基因组长98，392 bp，这使其成为迄今为止测序的最小的鳞翅目感染杆状病毒。其G + C含量为29.7%，编码125个推定的开放阅读框架（ORF）。结果表明，该病毒的37个核心基因和19个β-杆状病毒特异性基因全部被发现，其中13个基因在其他杆状病毒基因组中未发现。系统发育分析表明，DisaGV属于Betabaculovirus属，与ChocGV、ErelGV、PiraGV分离株、ClanGV、CaLGV、CpGV、CrleGV、AdorGV、PhopGV和EpapGV形成一个姐妹群。令人惊讶的是，我们在DisaGV基因组中发现了一个与鳞翅目和其他昆虫病毒基因相关的G蛋白偶联受体和一个gp 64同源物，这可能是第1组α杆状病毒水平基因转移的产物。DisaGV代表β杆状病毒属的独特谱系。它与CpGV相关组密切相关，并且迄今为止呈现最小的基因组。值得注意的是，我们发现了一个gp 64的同源物，这是迄今为止仅在组1 α杆状病毒基因组中报道的。本文的在线版本（doi：10.1186/s12864-016-2408-9）包含补充材料，可供授权用户使用。
A betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses. DisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far. The online version of this article (doi:10.1186/s12864-016-2408-9) contains supplementary material, which is available to authorized users.